ACQUISTION OF A CYTOMATION MOFLO CELL SORTER

此应用程序请求资金购买Cytomation MoFlo MLS2000高性能流式细胞仪。这台仪器将在一个标准的光学工作台上配置三个激光器、一个气溶胶遏制装置和一个高分辨率细胞沉积单元。MoFlo将成为华盛顿大学阿尔文·J·西特曼癌症中心内一个新的细胞分选核心设施的基础。在这项提案中确定的用户，所有由NIH资助的具有重要细胞学专业知识的知名科学家，将占到80%的使用。剩下的 时间将分配给华盛顿大学的其他调查人员，优先考虑Siteman癌症中心的成员。华盛顿大学或圣路易斯大学健康科学中心附近的校园目前没有高速细胞分选机。这台仪器将极大地帮助关键人员，他们的不同研究兴趣包括造血和祖细胞动员，白血病发生的分子机制，疱疹 病毒潜伏期，T细胞发育和激活，移植物抗宿主病，以及实体器官移植后的免疫反应。此外，Siteman癌症中心(其成员在2000财年从NIH获得7620万美元的与癌症相关的研究的直接费用)和整个华盛顿大学科学界将获得巨大的红利。核心设施将由私人侦查员直接监督，并配备一名经验丰富的全职技术人员。申请的资金仅用于购买MoFlo。这个 细胞分选核心设施的启动和运行费用将由该机构提供，并由用户费用抵消。一个咨询委员会将监督细胞分类器的管理，并确保其作为医学院资源的长期稳定性。

This application requests funds to purchase a Cytomation MoFlo MLS2000 high performance flow cytometer. This instrument will be configured with three lasers on a standard optical bench, an aerosol containment device, and a high resolution cell deposition unit. The MoFlo will be the foundation of a new cell sorting core facility within the Alvin J. Siteman Cancer Center at Washington University. The users identified in this proposal, all established NIH-funded scientists with significant cytometry expertise, will account for 80% of usage. The remaining hours will be allocated to other investigators at Washington University, with priority given to members of the Siteman Cancer Center. There are currently no high-speed cell sorters available at Washington University or the nearby campus of St. Louis University Health Sciences Center. This instrument will greatly benefit the key personnel, whose diverse research interests include hematopoiesis and progenitor mobilization, molecular mechanisms of leukemogenesis, herpes viral latency, T cell development and activation, graft-versushost disease, and the immune response after solid organ transplantation. In addition, there will be significant dividends for the Siteman Cancer Center (whose membership was awarded $76.2 million in direct costs for cancer-related research from the NIH in FY 2000) and the Washington University scientific community at large. The core facility will be directly supervised by the P.I. and staffed by an experienced, full-time technician. The funds requested are for purchase of the MoFlo only. The startup and operational costs of the cell sorting core facility will be provided by this institution and offset by user fees. An advisory committee will oversee management of the cell sorter and ensure its long-term stability as a resource for the medical school.