CORE--MOLECULAR ANALYSIS

核心B（分子分析）核心旨在提供集中的 用于定量PCR、DNA测序和序列分析的资源， 一组特征质粒。这些任务被确定为 重要的技术和资源，需要专业知识和昂贵的 一个实验室不具备的设备。的一个重点 分子核心将是促进定量PCR使用实时 由Applied Biosystems开发的荧光PCR“TaqMan”测定。是 重要的是测量载体和转基因表达的水平， 基因治疗实验的绝对单位，以回答 关于基因治疗效果的基本问题， 向量。核心技术人员将进行Taqman分析， 核心主任将与项目科学家合作， 战略和质量控制数据。此外，分子 分析核心将促进特征基因的构建 通过提供经验证的起始质粒集合 并通过使用自动荧光DNA分析新质粒 测序核心主任将提供信息支持， 与DNA序列的检索和分析相关的计算机软件， 用于各种应用和克隆的寡核苷酸的设计 战略布局

Core B (Molecular analysis) core is intended to provide a centralized resource for quantitative PCR, DNA sequencing and sequence analysis, and a collection of characterized plasmids. These tasks were identified as important techniques and resources requiring expertise and expensive equipment not available to a single laboratory. One focus of the molecular core will be to promote quantitative PCR using the real-time fluorescent PCR "TaqMan" assay developed by Applied Biosystems. It is important to measure the levels of vector and transgene expression in gene therapy experiments in absolute units in order to answer fundamental questions about the efficacy of gene therapy by various vectors. The core technician will perform the Taqman analyses and the core director will collaborate with the project scientists in developing strategies and quality controlling data. In addition, the molecular analysis core will promote the construction of well characterized gene therapy vectors by providing a verified collection of starting plasmids and by analysis of new plasmids using automated fluorescent DNA sequencing. The core director will provide informatic support in the use of computer software related to retrieval and analysis of DNA sequences, design of oligonucleotides for various applications and cloning strategies.