Core--Image analysis and bioassays

由于使用生物传感器和生物标记物来定义活细胞和组织功能的非侵入性成像工具的进步，德克萨斯A&M大学的超级基金基础研究项目在很大程度上依赖于图像分析实验室的设施和人员支持。五个不同的SBRP项目将利用该核心的分析成像技术来监测暴露于各种相关毒物的细胞内的许多重要功能端点。功能终点包括细胞内谷胱甘肽、pH值和Ca2+稳态、细胞间通讯和GFP标记蛋白表达的评估。额外的显微镜支持与数字图像采集和分析原位杂交和免疫细胞化学将提供。该核心将提供与现场服务和分析服务核心的接口，接收和/或分发分析服务核心的现场服务提供的样品给负责进行内分泌干扰物、遗传毒性或污染物敏感生物测定的研究人员。由于其被不同的研究项目和与其他设施核心的互动广泛使用，图像分析和生物分析核心对推进SBRP目标有着坚定的承诺，并作为SBRP研究者之间科学互动的论坛。

The Superfund Basic Research Program at Texas A&M University has relied heavily on the facilities and staff support available in the Image Analysis Laboratory due to advances in non-invasive imaging tools using biosensors and biomarkers for defining the function of living cells and tissues. Five different SBRP projects will exploit the analytical imaging technologies within this core to monitor numerous vital functional endpoints within cells exposed to a diverse array of relevant toxicants. Functional endpoints include assessment of intracellular glutathione, pH and Ca2+ homeostasis, intercellular communication, and GFP- tagged protein expression. Additional microscopy support with digital image acquisition and analysis for in situ hybridization and immunocytochemistry will be provided. This Core will provide the interface with the Field Services and Analytical Services Cores receive and/or distribute samples provided by the Field Services of Analytical Services Cores to investigators responsible for performing endocrine disruptor, genotoxicity, or pollutant-sensitive bioassays. Because of its extensive utilization by the different research projects and interactions with other facilities cores, the Image Analysis and Bioassays Core has a strong commitment to advancing the SBRP goals and serves as a forum for scientific interactions between SBRP investigators.