BINDING INTERACTIONS WITHIN PEPTIDE-MHC COMPLEXES

本研究的目的是确定肽与MHC II类蛋白之间结合相互作用的相对能量学。辅助性T细胞通过T细胞上的抗原受体与其他细胞表面表达的配体相互作用而被激活。这些配体是与MHC II类蛋白结合的肽复合物。肽结合在蛋白质远端膜表面的凹槽中。结合相互作用包括从MHC侧链到肽主链的一系列氢键，以及肽侧链所在的肽结合槽中的口袋。虽然对这些配合物的结构了解甚多，但不同结合相互作用对配合物整体稳定性的相对贡献并非未知。我们建议系统地消除各种肽- mhc复合物的特定氢键和口袋相互作用，并评估它们对稳定性的贡献。这些结果将定义肽与MHC II类蛋白结合的规则。这些结合规则将极大地帮助抗原肽- mhc复合物的分析和t细胞表位的鉴定。

The objective of this research is to define the relative energetics of binding interactions between peptides and MHC class II proteins. Helper T cells are activated by the interaction of an antigen receptor on the T cell with ligands expressed on the surface of other cells. These ligands are complexes of peptides bound to MHC class II proteins. The peptide is bound in a groove on the membrane distal face of the protein. Binding interactions include an array of hydrogen bonds from MHC sidechains to the peptide backbone and pockets in the peptide-binding groove on which peptide sidechains reside. While much is known about the structure of these complexes, the relative contributions of the different binding interactions to the overall stabilities of the complexes are not unknown. We propose to systematically eliminate specific hydrogen-bond and pocket interactions for a variety of peptide-MHC complexes and evaluate their contribution the stabilities. These results will define the rules for peptide binding to MHC class II proteins. The binding rules will greatly aid analyses of antigenic peptide-MHC complexes and the identification of T-cell epitopes.