NOVEL CLINICAL CHEMISTRY SENSING MOTIF

我们将开发新颖、简单、廉价的临床化学传感材料，它可以作为视觉颜色测试条，对Pb2+、K+、Hg2+、pH、Ca2+等重要分析物的浓度和身份进行敏感和选择性的报告。我们将开发廉价的临床试纸，它可以浸入体液中，在病人床边准确地测定分析物。我们还将开发这些化学传感材料的阵列，这些材料将附着在光纤束上，用作多通道光电极，同时监测许多分析物。这些传感材料是基于我们最近开发的智能聚合晶体胶体阵列技术。这种创新的传感基序利用了一种介观周期性排列的胶体颗粒聚合成丙烯酰胺水凝胶。由于胶体粒子的周期性排列，该阵列在可见光谱区域衍射光。该材料还包含为感兴趣的分析物量身定制的螯合剂。当分析物结合时，其电荷被固定在丙烯酰胺水凝胶内。由此产生的唐南电位引起渗透压，使水凝胶阵列与被分析物的浓度成比例地膨胀。这导致在衍射波长的变化和在感知颜色的变化。波长衍射的变化报告了目标分析物的身份和浓度。这个基序是高度灵活的，可以修改，以感知广泛的种类和浓度的生物相关分析物。

We will develop novel, simple, inexpensive clinical chemistry sensing materials which can be used as visual color test strips to sensitively and selectively report on the concentration and identity of important analytes such as Pb2+, K+, Hg2+, pH, Ca2+, etc. We will develop inexpensive clinical test strips which can be immersed in bodily fluids to accurately determine analytes at the patient bedside. We will also develop arrays of these chemical sensing materials which will be attached to fiber optic bundles to be used as multichannel optrodes to simultaneously monitor numerous analytes. These sensing materials are based on the intelligent polymerized crystalline colloidal array technology we recently developed. This innovative sensing motif utilizes a mesoscopically periodic array of colloidal particles polymerized into an acrylamide hydrogel. This array diffracts light in the visible spectral region due to the periodic array of colloidal particles. This material also contains chelating agents tailored for the analytes of interest. When an analyte binds, its charge is immobilized within the acrylamide hydrogel. The resulting Donnan potential causes an osmotic pressure, which swells the hydrogel array in proportion to the concentration of analyte bound. This results in shifts in the diffracted wavelength and in the perceived color changes. The change in the wavelength diffracted reports on the identity and concentration of the target analyte. This motif is highly flexible and can be modified to sense a broad variety and concentration of biologically relevant analytes.