STUDIES OF MOLYBDENUM CONTAINING ENZYMES

本文对四种相关的单核钼酶：黄嘌呤氧化还原酶、亚硫酸盐氧化酶、DMSO还原酶和亚砷酸盐还原酶的基本酶学性质和光谱性质进行了全面的研究。这一组包括所有三个主要的单核钼酶家族的代表。拟议工作的总体目标是通过比较和对比这些酶的物理、化学和电子结构，更完整地了解每种酶的作用机制的基本方面。还将就结构与功能的关系对不同类型的酶进行比较。为此，将直接在PI实验室过去工作的基础上进行一系列快速反应动力学、光谱、理论和分子生物学研究。拟议的工作旨在将这些酶的机理和光谱研究与其已知的晶体结构相结合，并通过比较和对比它们的性质来阐明反应活性的潜在主题。这一方法在对具有血红素和黄素假体基团的通常被更好地理解的生物系统的研究中被证明非常成功。

The present application involves a comprehensive investigation of the basic enzymological and spectroscopic properties of four related mononuclear molybdenum enzymes: xanthine oxidoreductase, sulfite oxidase, DMSO reductase and arsenite reductase. This group includes representatives of all three major families of mononuclear molybdenum enzymes. The overall goal of the proposed work is to gain a more complete understanding of fundamental aspects of the mechanism of action of each of these enzymes by comparing and contrasting their physical, chemical and electronic structures. A comparison among enzymes type with regard to the relationship of structure to function will also be made. To this end a series of rapid reaction kinetic, spectroscopic, theoretical and molecular biological studies will be undertaken that build directly on past work in the PI's laboratory. The proposed work is intended to integrate both mechanistic and spectroscopic studies of these enzymes with their known crystal structures and to elucidate underlying themes in reactivity by comparing and contrasting their properties. This approach that has proven extremely successful in studies of the generally much better understood biological systems that possess heme and flavin prosthetic groups.