ATTEMPTED MEASURE OF THREE PHOTON EXCITATION CROSS SECTION OF DIHYDROERGOSTEROL

在过去的几年里，我们一直在与 光谱物理激光器对超快激光器的要求 多光子显微镜。我们过去的合作导致了 蓝光反射镜的研制，波长可低至 680 Nm将与海啸、光谱-物理商业TIS一起进入 激光。目前我们正在两个领域进行合作，这两个领域将 促进生物学家采用多光子显微镜(1) 极宽调谐镜组(700~900)的研制 Nm)，消除了在组之间更换镜像的需要，以及(2) 最小波长集的规范将是 如果在单盒(固态泵)中实施，则适用于MPM 包括)，交钥匙系统。这样的系统将被设计成 仅在3或4个预设波长下工作，以简化激光操作。

Over the past few years we have been in consultation with Spectra-Physics Lasers on the requirements of ultrafast lasers used in multiphoton microscopy. Our past collaboration has lead to the development of the "blue" mirror set that allows wavelengths as low as 680nm to be accessed with the Tsunami, Spectra-Physics commercial TiS laser. Currently we are collaborating in two areas which will facilitate the adoption of multiphoton microscopy by biologists (1) the development of an extremely broad tuning mirror set (700 to >900 nm) which eliminates the need to change mirrors between sets, and (2) the specification of the minimal set of wavelengths that would be suitable for MPM if implemented in a single box (solid-state pump included), turnkey TiS system. Such a system would be designed to operate at 3 or 4 preset wavelengths only to simplify laser operation.