CORE--SCIENTIFIC

科学核心的目的是提供各种 转基因和敲除突变体，原代细胞培养，一般 分子生物学技术、生化分析和形态学 和组织学方法的四个项目的程序。的 这些服务的可用性将使调查人员能够进行 他们的研究与各种方法的质量控制一致 和分析的方式，这将是不可能的个别项目 实现。这一科学核心的一个特殊优势是， 项目负责人积极参与执行 实验，并提供自己的专业知识，以他人在每月 研究研讨会会议。例如，陈博士（核心领导人）将 提供他的专业知识，在使用超氧化物歧化酶转基因， 基因敲除突变小鼠以及氧自由基相关的生物化学 和分子生物学。夏普博士将提供他在基因方面的专业知识 原位杂交和免疫细胞化学 放射自显影和[14 C]碘安替比林放射自显影 血流诺布尔博士将提供她在形态学方面的专业知识 评估（光和超微结构）神经元损伤。博士 Swanson将提供他在.OH自由基HPLC测量方面的专业知识 和H2 O2以及使用成像定量测量梗死体积 分析.此外，Sylvia Chen博士， 科学核心，将提供她在分子分析的专业知识， 基因表达（PCR，RT-PCR），原代细胞培养和各种 生化分析因此，这个科学核心提供了一个独特的 各种项目的工具，以实现其研究目标， 统一的方法和专门知识，将相互补充。

The purpose of the Scientific Core is to make available various transgenic and knockout mutants, primary cell cultures, general molecular biological techniques, biochemical assays, and morphological and histological methods to the four projects of the program. The availability of these services will allow investigators to conduct their studies with consistency in quality control of various methods and assays in a way that would not be possible for individual projects to achieve. One particular strength of this Scientific Core is that all the project leaders are actively participating in the execution of the experiments and provide their own expertise to others during monthly research seminars meetings. For example, Dr. Chan (Core Leader) will provide his expertise in the use of superoxide dismutase transgenic and knockout mutant mice as well as oxygen radicals-related biochemistry and molecular biology. Dr. Sharp will provide his expertise in gene expression (in situ hybridization and immunocytochemistry), DG autoradiography and [14C iodoantipyrine autoradiography for cerebral blood flow. Dr. Noble will offer her expertise in morphological assessments (both light and ultrastructure) of neuronal injury. Dr. Swanson will offer his expertise in HPLC measurements of .OH radicals and H2O2 and quantitative measurements of infarct volume using imaging analysis. In addition, Dr. Sylvia Chen, the coordinator of the Scientific Core, will provide her expertise in molecular analysis of gene expression (PCR, RT-PCR), primary cell culture and various biochemical assays. Thus, this Scientific Core provides an unique vehicle for various projects to achieve their research goals with a unified methodology and expertise that will complement one another.