CORE--VECTOR 1--ADENOVIRUS/ADENO ASSOCIATED VIRUS HERPESVIRUS LIPOSOME

核心A(向量1)将在设计中提供帮助， 基因治疗载体的构建及结构验证 基于腺病毒(Ad)、腺相关病毒(AAV)、疱疹 单纯疱疹病毒1型(HSV)。核心的主要作用是提供 知识和生物材料给调查人员，这样他们就可以 把重点放在项目的生物学方面，而不是 基因治疗技术。出于这个原因，核心将履行 以下角色：执行大规模载体准备工作 参与项目(目标为50个筹备/年) 利用可用的核心使用分配和资源；创建新的 通过引进技术和技术的改进来实现基因治疗载体 /或对参与者来说很重要的新转基因 项目(目标18/年)；提供标准操作程序 对于构建所有三种类型的病毒的所有阶段 向量；通过教程进行向量构造的教育 高级核心科学家和跟踪核心技术人员；供应 用于载体构建的经过验证的生物材料样本 包括质粒、细胞系和起始载体，并保持 描述这些材料的数据库。

Core A (Vector 1) will provide assistance in the design, construction and struCtural verification of gene therapy vectors based on adenovirus (Ad), adeno-associated virus (AAV), herpes simplex virus I (HSV). The major role of the core is to provide knowledge and biological materials to the investigators so they may focus on the biological aspects of their projects rather than the technology of gene therapy. For this reason the core will fulfill the following roles: Perform large scale vector preparations for the participating projects (target 50 preparations /year) in accordance with the core usage allocations and resources available; Create new gene therapy vectors by importing improvements in the technology and / or new transgenes that are important for the participating projects (target 18 / year); Provide Standard Operating Procedures for all phases in the construction of all three types of viral vector; Education in construction of vectors through tutorials with senior core scientists and shadowing core technicians; Supply verified samples of biological materials for the vector construction including plasmids, cell lines and starting vectors, and maintain a database describing those materials.