THE ROLE OF SEQA PROTEIN IN E.COLI REPLICATION CONTROL

这项研究计划的长期目标是确定DNA复制速度如何与大肠杆菌模式生物的细胞分裂周期相协调。了解细菌中的这些事件对于指导未来人类细胞周期研究至关重要。为了实现这一目标，本研究项目旨在解决有关大肠杆菌复制控制的两个问题：第一，SEQA蛋白在调控DNA复制启动中扮演什么角色，具体地说，它在控制细胞内起始点移动方面扮演什么角色？第二，在部分受SEQA控制的复制过程中发生的一系列事件与复制开始的时间之间有什么关系？这些问题将通过检查各种事件的发生时间、荧光显微镜和分子方法，以及在复制启动控制方面存在缺陷的突变细胞来解决。在这次调查的过程中，我们将开发一种新的方法来获得同步培养。

It is the long-term goal of this research proposal to determine how the rate of DNA replication is coordinated to the cell division cycle in the model organism of E. coli. Understanding these events in bacteria is essential to guide future cell-cycle research in humans. To achieve this goal, this research project is designed to resolve two questions about replication control in e. COLI: First, what is the role of SeqA protein in regulating initiation of DNA replication, and specifically, what is its role in controlling origin movement within the cell? Second, what is the relationship between the sequence of events occurring at replication termiantion in part controlled by SeqA, and the timing of replication initiation? These questions will be addressed by examining the timing of varius events, by fluorescence microscopy and molecular methods, that occur at and immediately and mutant cells that are defective in the control of replication initiation. In the process of this investigation, we will develop a new method to obtain synchronized cultures.