GENOME ANALYSIS OF STREPTOCOCCUS GORDONII

描述(调查者？S摘要)：本项目的目标是确定 先锋定殖者戈登链球菌的全基因组序列 牙菌斑和感染的重要病原菌 心内膜炎。牙菌斑的形成受复合体的调节 宿主的牙齿表面与表面结构之间的相互作用 戈登葡萄球菌和其他口腔细菌。相互作用面的基础 结构是高度特定的黏附机制，由 粘附素受体伙伴关系和细胞外葡聚糖。感染性心内膜炎 通常由于口腔创伤而发生，可能依赖于 产生蛋白水解酶和额外的毒力因子。基因重组 和转移粘附素，抗生素耐药性决定因素和可能的 口腔链球菌之间的毒力因子可能是进化的原因 粘附素/受体对与细菌耐药性的关系 戈登氏链霉菌S.gordonii.最后，戈登葡萄球菌已被证明是一种有效的 疫苗研发的抗原输送载体。 通过对基因组结构的鉴定，新的黏附/受体和 监管要素，该项目将加快定向的实验工作 了解戈登葡萄球菌在牙菌斑形成中的动态 以及它在感染性心内膜炎中的作用。排序的方法 戈登链球菌Challis(NCTC7868)将是一个改良的整体 基因组随机测序策略在TIGR成功应用 原核生物基因组序列14。该项目将分为四个阶段：1) 随机中、小插入质粒库的构建及大片段的构建 插入Gordonii菌株Challis的BAC文库，2)两端测序 大约23,500个中小型插入克隆，3)测序 一组重叠最少的BAC克隆，以提供脚手架结构 这将最大限度地减少缝隙关闭所需的工作量，并提供确认 基础装配结构的装配和批注，以及4) 基因组识别结构特征，分配基因和功能角色 基于数据库相似性搜索的开放阅读框架。开发的数据 来自这项研究的数据将储存在几个数据库中，包括TIGR网站 地点。此外，所有克隆集都将提供给研究人员 社区。

DESCRIPTION (Investigator?s Abstract): The goal of this project is to determine the complete genome sequence of Streptococcus gordonii, a pioneering colonizer of dental plaque and significant causative bacterium for infective endocarditis. The formation of dental plaque is regulated by complex interactions between the dental surfaces of the host and the surface structures of S.gordonii and other oral bacteria. The basis of the interacting surface structures is highly specific adherence mechanisms controlled by adhesin-receptor partnerships and extracellular glucans. Infective endocarditis often occurs as a result of oral trauma and is likely dependent upon the production of proteases and additional virulence factors. Genetic recombination and transfer of adhesins, antibiotic resistance determinants and possible virulent factors between the oral streptococci may be responsible for evolution of adhesins/receptor pairs and development of antibiotic resistance in S.gordonii. Finally, S.gordonii has been shown to be an effective antigen-delivery vehicle for vaccine development. Through the identification of genome structure, novel adhesions/receptors and regulatory elements, this project will accelerate experimental work directed towards understanding the dynamics of S. gordonii in dental plaque formation and its role in infective endocarditis. The approach to sequencing Streptococcus gordonii strain Challis (NCTC7868) will be a modified whole genome random sequencing strategy successfully used at TIGR to completely sequence 14 prokaryotic genomes. The project will consist of four phases: 1) construction of random small and medium insert plasmid libraries and a large insert BAC library from S. gordonii strain Challis, 2) sequencing both ends of approximately 23,500 small and medium insert clones, 3) sequencing the ends of a set of minimally overlapping BAC clones to provide a scaffolding structure that will minimize the effort required for gap closure and provide confirmation of the underlying assembled structure, and 4) assembly and annotation of the genome to identify structural features, assign gene and functional roles to open reading frames based upon database similarity searches. The data developed from this study will be deposited in several databases, including the TIGR web site. In addition, all clone sets will be made available to the research community.