GENETIC BASIS OF SPECIES DIFFERENCES IN DROSOPHILA

描述：PI计划继续他非常成功的研究计划 关于物种形成的遗传基础。这项基于果蝇的研究已经 主要集中在亲缘关系非常密切的物种之间的差异 可能在物种隔离中起到了重要作用。尤其是，很多 这项拟议工作的动机是越来越多的证据表明 合子隔离可能是最常见和最早出现的形式 物种形成事件的隔离。与基因相关的表型研究 最近的物种形成事件可能很复杂。一类表型是 作为进化的一个组成部分可能出现的物种差异 生殖隔离。通常，这些差异是受性别限制的，因此 在这种情况下，至少有四类生物需要研究 (雄性和雌性，每两个物种)。第二类表型 是生物体对的特征(例如，任何与前- 合子对杂交性行为的限制，如配偶选择)，有两个 可以被认为是每一对兄弟物种的互惠对。 此外，这些表型通常必须与它们的 内部特定形式。第三个班级至少包括两个有趣的 只在物种杂交中表现出来的表型，不育和 无法生存。这些可以在两个正反交组合中进行研究，潜在地， 取决于杂交种的不生存能力和可避免的遗传工具 它。拟议的研究将集中在这三种类型中的每一种。 分类，每个分类都要遵守基因图谱协议。

DESCRIPTION: The PI plans to continue his very successful research program on the genetic basis of speciation. This Drosophila based research has primarily focused on differences between very closely related species that may have been important in the isolation of species. In particular, much of the proposed work is motivated by a growing body of evidence that pre- zygotic isolation may be the most common and first arising form of isolation for speciation events. The study of phenotypes associated with recent speciation events can be complex. One class of phenotypes are species differences that may have arisen as a component of the evolution of reproductive isolation. Often these differences are sex-limited so that there are at least four classes of organisms to examine in this context (males and females in each of two species). A second class of phenotype are features of pairs of organisms (e.g. anything associated with pre- zygotic limits to hybrid sex, such as mate choice) and there are two reciprocal pairs that can be considered for each pair of sibling species. Also these phenotypes must usually be measured in comparison to their intra-specific form. A third class includes at least two interesting phenotypes that are only manifest in species hybrids, sterility and inviability. These can be studied in both reciprocal crosses, potentially, depending on hybrid inviability and the genetic tools available to avoid it. The proposed research will focus on phenotypes in each of these three categories, subjecting each to a gene mapping protocol.