FLOW IN THICK TISSUES PROBED BY DIFFUSING LIGHT

描述(改编自申请人摘要)：浓度， 血细胞在微血管系统中的氧合和流动特性 深刻地影响周围组织的营养和热调节。如果 这些血液变量变得异常，那么组织的健康可能会受到威胁。 对这些基本血液参数的测量可以揭示严重的 烧伤、重建手术、周围血管疾病(PVD)、脑 代谢和功能异质性，内出血，肿瘤血管， 淋巴流动和缺血。这项竞争延续拨款的目的是为了 改进、验证和应用漫反射光谱学进行研究 深部组织的血液动力学。已经建立了新的仪器， 首次将光子相关技术用于组织研究 内部运动，如血流，带有漫反射光子密度波 用于研究组织吸收和散射的光谱工具。新的 仪器使血液流动、血液的无创分离成为可能 氧合散射和血容量组织反应。而当 到目前为止的实验结果是有用的，并揭示了最终的希望 在研究和临床应用中，申请人确定了几个 继续取得进展对实现这一目标至关重要的目标领域 方法学从发展阶段走向临床应用。这个 拟议的研究有三个相互关联的组成部分。一个理论组成部分 针对申请者计划的生理问题开发更好的模型 在小鼠肿瘤、大鼠脑和人类四肢上进行研究。一秒钟 组件在定义良好的组织中验证仪器和分析 幻影和生理模型，其生物反应是 很好地理解和/或用另一种实验技术测量。这个 最后一部分研究了复合光学材料的可转移性 诊断学可用于：(1)最先进的肿瘤动物研究 (2)正在进行的临床研究 外周血管反应。

DESCRIPTION (Adapted from Applicant's Abstract): The concentration, oxygenation, and flow characteristics of blood cells in the microvasculature profoundly affect nourishment and thermal regulation of surrounding tissues. If these blood variables become abnormal, then tissue health may be threatened. Measurement of these basic blood parameters can reveal information about severe burns, reconstructive surgeries, peripheral vascular disease (PVD), brain metabolism and functional heterogeneity, internal bleeding, tumor vasculature, lymph flow, and ischemia. The goal of this competing continuation grant is to improve, validate and apply diffuse optical spectroscopy for the investigation of blood dynamics in deep tissues. New instrumentation has been built that combines for the first time photon correlation technology for studies of tissue internal motions such as blood flow, with diffuse photon density wave spectroscopic tools for studies of tissue absorption and scattering. The new instrument makes possible noninvasive separation of blood flow, blood oxygenation scattering, and blood volume tissue responses. While the experimental results to date are useful and reveal promise for eventual research and clinical application, the applicants have identified several target areas wherein continued progress is critical to transfer this methodology from the developmental stage toward clinical applications. The proposed research has three interrelated components. A theoretical component develops better models specific to physiological problems the applicants plan to investigate in murine tumors, rat brain, and on human limbs. A second component validates the instruments and analysis in well defined tissue phantoms and physiological models for which the biological response is either well understood and/or is measured with another experimental technique. The last component investigates how transferability of the composite optical diagnostics can be used in: (1) State-of-the-art animal studies of tumor necrosis factor and cerebral metabolic rate, and (2) Ongoing clinical studies of peripheral vascular response.