MICROMASS Q-TOF MASS SPECTROMETER

约翰霍普金斯大学（JHU）的许多研究人员迫切需要获得常规的高灵敏度质谱分析能力。基础科学部门领导在行政部门的大力支持下，正在建立一个真正的开放式的，最先进的质谱设备。在该计划的第一阶段，几个主要用户、院长办公室和生物化学部共同购买了一台Perseptive MALDI-TOF DE仪器。作为第二阶段的一部分，请求提供资金购买Micromass Q-TOF电喷雾飞行时间质谱仪。这两种类型的MS的互补能力将极大地促进JHU许多不同的NIH支持的研究项目的进展。Q-TOF MS的独特功能对于许多此类研究至关重要。MS设施和Q-TOF将由具有广泛MS专业知识的讲师级教员指导。MALDI-TOF将继续由经验丰富的全职技术人员操作/监督。私家侦探和联合私家侦探将至少每周与工厂工作人员会面，讨论技术问题、工作量和优先事项。一个由私家侦探组成的监督委员会，副PI，JHU蛋白质设施的主任，和三个部门。主任也是主要用户，他们将每月开会讨论政策和问题。将在第一年年底寻求外部专家审查。工作人员支助将至少维持在这一水平。仪器将在其使用寿命内保持全面服务协议。MALDI-TOF的使用费为50美元/小时，Q-TOF MS-MS的使用费为100美元/样本（包括数据分析），将退还给机构。MS设施将为110多个NIH资助的实验室提供服务。确定了10个主要用户和10个典型的次要用户。优先级将由时间顺序决定，但主要用户优先。为期一周的MS课程将提交给教师，学生和博士后。伴随着设施的开放。

Many investigators at Johns Hopkins (JHU) have urgent need for routine access to high sensitivity mass spectrometric analytical capabilities. The Basic Science departmental leadership, with the strong support of the Admin., are establishing a true open-access, state-of-the-art, mass spectrometric facility. In phase I of this plan, several major users, the Dean's office, and Biological Chemistry have collectively purchased a Perseptive MALDI-TOF DE instrument. As part of phase II, funds are requested to purchase a Micromass Q-TOF electrospray time of flight mass spectrometer. The complimentary capabilities of these two types of MS will greatly enhance progress of many varied NIH supported research programs at JHU. The unique capabilities of the Q-TOF MS are essential to many of these investigations. The MS Facility and the Q-TOF will be directed by an Instructor level faculty member with extensive MS expertise. The MALDI-TOF will continue to be operated/supervised by an experienced full-time technician. The P.I. and co-P.I. will meet at-least weekly with the facility staff to discuss technical issues, workload, and priorities. An oversight committee comprised of the P.I., co-P.I., the Director of the JHU protein facility, and three Dept. Directors, who are also major users, will meet monthly to discuss policy and problems. Outside expert review will be sought at the end of the first year. Staff support will be maintained at at- least this level. Instrumentation will be kept on full service agreements for their useful lifespans. Use charges of $50/h for MALDI-TOF and $100/sample for Q-TOF MS-MS (includes data analysis) will be returned to the facility. The MS facility will service over 110 NIH-funded laboratories. Ten major and ten typical minor users are identified. Priority will be determined by chronology, but with major users given precedence. A week-long MS course will be presented to faculty, students, and postdoc. fellows concomitant with the facility opening.