FACS VANTAGE SE CELL SORTER/FLOW CYTOMETER

这是根据PAR-99-031申请的共享仪器赠款，寻求295,649美元的资金，用于购买最先进的细胞分选机/流式细胞仪，以更换已有14年历史和报废的流式细胞仪。该仪器将被安置在田纳西诺克斯维尔大学兽医学院设备齐全的核心设施中。该仪器需要支持田纳西诺克斯维尔大学兽医学院的3名NIH资助的研究人员(Rouse、Schuller和Wang博士)和来自田纳西诺克斯维尔医学中心大学的1名NIH资助的合作者(Wimalasena博士)的研究。这些调查员目前正在进行的和待批的赠款在很大程度上取决于这一文书的可得性。所附的Becton Dickinson报价是FACS Vantage TM/SE细胞分选机，配有Argon UV激光器、氦-霓虹灯激光器、脉冲处理器、荧光探测器、第三台激光支架和INDO-1过滤器组件。如果获奖，其他供应商将有机会与贝顿·迪金森竞争。描述了长期财务计划设施主任、技术设施经理和咨询委员会。

This is an application for a shared instrumentation grant under PAR-99- 031 seeking funds in the amount of $295,649 for the purchase of a state of the art cell sorter/flow cytometer to replace a 14 year old and defunct flow cytometer. This instrument will be housed in a fully equipped core facility located in the College of Veterinary Medicine, University of Tennessee Knoxville. This instrument is needed to support the research of 3 NIH funded investigators at the College of Veterinary Medicine, University of Tennessee Knoxville (Drs. Rouse, Schuller, and Wang) and 1 NIH funded collaborator from the Medical Center University of Tennessee, Knoxville (Dr. Wimalasena). The currently active and pending grants of these investigators depend heavily on the availability of this instrument. The attached quotation from Becton Dickinson is for a FACS Vantage TM/SE Cell Sorter with Argon UV laser, helium-neon laser, pulse processor, fluorescence detection, third laser mount, and INDO- 1 filter- set. If an award is made, other vendors will be given the opportunity to bid against Becton Dickinson. A long-term financial plan facility director, technical facility manager, and advisory committee are described.