EPICS XL-MCL FLOW CYTOMETRY SYSTEM

这项提议的目的是获得一种分析性的流式细胞仪，供血液研究中心(CBR)的研究人员共享，以开展专注于血液研究各个方面的现有研究项目和新的研究项目。目前将专门利用这一仪器的项目有：1)一个三组分的项目，重点是血细胞信号通路和细胞骨架组织(弗雷德·S·罗森博士)，(2)免疫和宿主防御的遗传和细胞机制研究(切斯特·A·阿尔珀博士)，(3)艾滋病毒感染疾病中T淋巴细胞功能的研究(Wiskott-Aldrich综合征)(艾琳·雷莫尔德-O-唐奈博士)。分析流式细胞术是所有这些项目的核心技术手段，由NIH-PEER资助，并将用于各种细胞和分子生物学模型系统，以研究血细胞表面和细胞内蛋白质的功能。这些正在进行的研究的结果将有助于更好地理解免疫、宿主防御和细胞分化所涉及的分子机制，并将提供控制关键免疫学和血液学功能的机制的基本信息。所要求的库尔特XL型流式细胞仪系统将提高这些已建立和正在扩展的研究项目的生产率。该仪器是最先进的，具有满足血液研究科学家通过多功能和精细的流式细胞仪技术研究血细胞结构和功能的日益增长的应用的功能。该仪器能够在单个样品中分析多达四个不同标记的荧光探针，从而提高了分析的准确性，并减少了分析所需的成本、时间和样本量。IBM兼容的采集和分析计算机程序将允许自动化、高通量分析和专门的、精炼的高级应用程序，例如细胞内抗原的动力学测量和定量所需的高级应用程序。

The objective of this proposal is to acquire an analytical flow cytometry which will be shared by investigators at the Center for Blood Research (CBR) to conduct established and new research projects focused on various aspects of blood research. The current projects that will specifically utilize this instrument are: 1) a three-component program project focused on blood cell signaling pathways and cytoskeletal organization (Dr. Fred S. Rosen), (2) studies of the genetic and cellular mechanisms of immunity and host defense (Dr. Chester A. Alper), (3) studies of T lymphocyte function in HIV infection disease, the Wiskott- Aldrich syndrome (Dr. Eileen Remold-O-Donnell). Analytical flow cytometry us a central technical approach for all of this projects, which are funded by NIH-peer received grants, and will be used in a variety of cell and molecular biological model systems to investigate the function of blood cell surface and intracellular proteins. The results derived from these ongoing investigations will lead to a better understanding of the molecular mechanisms involved in immunity, host defense and cell differentiation and will provide fundamental information on the mechanisms that control key immunological and hematological functions. The requested Coulter Model XL flow cytometry analyzer system will enhance the productivity of these established and expanding research projects. This instrument is state-of-the-art and has features that meet the growing applications by blood research scientists to studies of the structure and function of blood cells by versatile and refined flow cytometry techniques. This instrument is capable of analyzing up to four differently labeled fluorescent probes in a single sample which increases the accuracy and decreases the cost, time and sample size required for analysis. The IBM compatible acquisition and analysis computer programs will allow both automated, high throughput analyses and specialized, refined advanced applications such as are required for kinetic measurements and quantitations of intracellular antigens.