ZEISS LSM 510 CONFOCAL MICROSCOPE

要求用蔡司LSM 510共聚焦显微镜取代目前已淘汰的W. M.怀特黑德研究所的凯克成像设备。该系统是由Zeiss、BioRad和Leica演示系统的用户现场评估后选择的。作为成像设施中共享仪器的一部分，蔡司共聚焦将直接支持WI和MIT的八个实验室的NIH支持的研究。此外，其他NIH支持的团体将可以访问新系统。共聚焦显微镜由配备荧光和DIC光学器件的Zeiss Axiovert显微镜、耦合到三个激光照明器的集成扫描单元和使用基于Windows NT的软件控制显微镜操作、图像采集和图像处理的Pentium PC组成。在稍后的时间，该系统将升级到多光子光学使用WI资金。怀特黑德研究所承诺支付10万美元的购买价格。维护和用户支持费用由WI预算支付。

A Zeiss LSM 510 confocal microscope is requested to replace the current but obsolete MRC 600 confocal microscope in the W. M. Keck Imaging Facility at the Whitehead Institute. This system was chosen after on-site evaluation by users of demo systems by Zeiss, BioRad, and Leica. As part of the shared instrumentation in the Imaging Facility, the Zeiss confocal will directly support the NIH-supported research of eight labs in the WI and MIT. In additional other NIH-supported groups will have access to the new system. The confocal microscope consists of a Zeiss Axiovert microscope equipped with fluorescence and DIC optics, an integrated scanning unit coupled to three laser illuminators, and a Pentium PC that controls the operation of the microscope, image acquisition, and image processing with a Windows NT-based software. At a later time, the system will be up-graded to multiphoton optics using WI funds. The Whitehead Institute commits $100,000 to defray the purchase price. The maintenance and user support costs are covered by a WI budget.