FACSCALIBUR FLOW CYTOMETER SYSTEM

分子医学和免疫学中心（CMMI）的主要工作重点是在患者和动物模型中进行癌症的免疫诊断和免疫治疗。在中心进行的个别项目都需要流式细胞仪的支持。该中心有一台1989年购买的Becton Dickinson FACScan。在过去的十年中，该仪器得到了中心所有研究人员的持续大量使用。自购买FACScan以来的十年中，流式细胞术技术取得了长足的进步，使今天的仪器在生产力和性能方面都更加优越。此外，我们对流式细胞术的需求在这段时间内也有所增长。因此，我们正在申请收购FACSCaliber流式细胞仪系统和FACSLoader，这是一种更新的流式细胞仪系统，将具有更高的可靠性，效率和更广泛的科学应用。流式细胞术在中心的具体用途包括：淋巴细胞表型，筛选细胞系与单克隆抗体的反应性，根据与肿瘤细胞系和/或正常细胞的反应性或缺乏反应性选择单克隆抗体，DNA细胞周期分析，凋亡研究，干细胞制剂中肿瘤细胞的检测，树突状细胞的分析，以及用于治疗研究中毒性评估的绝对细胞计数。该仪器的行政/科学监督，包括对内部工作人员的日常管理和技术培训，将由Stein博士在中心基础研究委员会的指导下负责。我们估计，主要用户将需要大约占该仪器总使用量的75-80%。此外，该仪器将提供给CMMI内部不太频繁的用户以及CMMI之外的用户，包括距离CMMI很近的罗格斯纽瓦克大学生物科学系的成员。

A major focus of the work at the Center for Molecular Medicine and Immunology (CMMI) is immunodiagnosis and immunotherapy of cancer, in patients and in animal models. The individual projects performed at the Center all require flow cytometry support. The Center has a Becton Dickinson FACScan, which was purchased in 1989. The instrument has received continuous heavy usage over the past decade by all investigators at the Center. During the ten years since the FACScan was purchased flow cytometry technology has advanced considerably, making the instrument available today much superior in terms of productivity and performance. In addition, our needs for flow cytometry have grown during this time period. Thus, we are submitting this application for the acquisition of the FACSCaliber Flow Cytometer System with the FACSLoader, an updated flow cytometry system, which will have greater reliability, efficiency, and broader scientific applications. Specific uses of flow cytometry at the Center include: lymphocyte phenotyping, screening cell lines for reactivity with MAbs, selection of MAbs based on reactivity or lack of reactivity with tumor cell lines and/or normal cells, DNA cell cycle analysis, apoptosis studies, detection of tumor cells in stem cell preparations, analyses of dendritic cells, and absolute cell counting for toxicity evaluations in therapy studies. Administrative/scientific oversight for the instrument, including day-to- day management and technical training for in-house staff will be the responsibility of Dr. Stein, under advisement of the Center's Basic Research Committee. We estimate that the major users will require approximately 75-80% of the total usage of the instrument. In addition, the instrument will be available to less frequent users within CMMI as well as users outside of CMMI, including members of the Biological Science Department at Rutgers Newark which is located a short distance from CMMI.