MOFLO TM MULTI-LASER FLOW CYTOMETER

这项申请是为了获得在康涅狄格大学健康中心(UCHC)购买新的荧光激活细胞分类器的资金。这种仪器名为MoFlo by Cytomation，将成为已经高度活跃的核心流式细胞仪的一部分。我们目前的仪器已经过时，也跟不上需求。这在一定程度上是由于最近和正在进行的NIH资助的教师招聘。在过去的两年里，加州大学洛杉矶分校成立了三个新的系/中心：免疫治疗中心、微生物发病中心和遗传与发育生物学系。所有三位中心主任都深度参与了流式细胞术，并参与了这项提议：遗传学新任主席Marc Lalande博士在流式细胞术(如染色体分类)方面拥有丰富经验，是该中心的一项宝贵资产。此外，每个中心将招聘几名新教师，其中许多将需要在他们的研究中使用流式细胞仪。我们最近还招募了一名干细胞免疫学家(H.L.阿吉拉，他与斯坦福大学的I.Weissman一起接受培训)，他的NIH资金悬而未决，他将需要大量使用MoFlo。此外，除了新的实验室，NIH资助的其他几个组织也对新的流式细胞仪技术有基本需求。如申请中所述，MoFlo将允许比UCHC目前提供的更快速、更高效的分选和更复杂的分析能力。这反过来将增加我们正在进行的和未来由NIH资助的项目的成功机会。

This application is to obtain funding for the purchase of a new fluorescence activated cell sorter at the University Of Connecticut Health Center (UCHC). This instrument, the MoFlo by Cytomation, would become part of an already highly active core flow cytometry facility. Our present instrument is outdated and also unable to keep up with demand. This is due in part to the recent and ongoing recruitment of NIH-funded faculty. Three new Departments/Centers have been created at UCHC in the last two years: the Center for Immunotherapy, the Center for Microbial Pathogenesis, and the Department Of Genetics and Developmental Biology. All three of the center directors are heavily involved in flow cytometry and are part of this proposal: Dr. Marc Lalande, new Chair of Genetics, has much experience in flow cytometry (e.g. sorting of chromosomes) and is a valuable asset to the facility. In addition, each center will be recruiting several new faculty, and many of these will require flow cytometry in their research. We have also recently recruited a stem cell immunologist (H.L. Aguila who trained with I. Weissman, Stanford) who has pending NIH funding and who will require significant use of the MoFlo. Furthermore, besides the new laboratories, several other NIH funded groups also have essential needs for new flow cytometry technology. As described in the application, the MoFlo will allow much more rapid and efficient sorting and more complex analytical power than is presently available at UCHC. This in turn will increase the chances of success for our ongoing and future NIH funded programs.