BIOSENSOR FOR THE DETECTION OF CONTAMINANTS IN FOOD

在这个项目中，我们将提供手持设备的原理验证， 专门用于检测和筛选的现场生物传感器 食品加工、制备不同阶段食品中的污染物 和处理中。多分析、多用途LAI生物传感器具有 能够进行定性和定量的测量、检测和 食品中食源性致病菌和毒素的筛查。生物传感器通过以下方式工作 感测直接结合，不需要二次荧光 报告分子，或其他中间化学反应。结果是 对生物传感器的性能进行了分析和仿真 显示了其在实时检测和筛选方面的潜力， 提高了检测灵敏度。该生物传感器可以被配置为便携式 “计算器大小”的单位，操作简单的特点。 由于其潜在的便携性，在不依赖供应的情况下 可现场使用的单元将用于州和地方水质污染 用于测试、过程控制和事件检测的检测。这些用途 提供了一个重要的市场。 建议的商业应用： 我们期望一个“实时”的便携式多病原体检测系统直接用于大型 规模化食品加工将提供行业所需的机制，以保持 以更低的人力和供应成本提高效率。同时转过身来 时间将大大减少与污染事件相关的潜在责任。其他 商业应用包括水质、病原体污染检测 市和州的水质计划。

In this project we will provide proof-of-principle validation of a handheld, fieldable biosensor specifically for the detection and screening of contaminants in foodstuffs at the various stages of food treatment, preparation and processing. The multi-analyte, multi-application LAI Biosensor has the capability for both qualitative and quantitative measurement, detection and screening of foodborne pathogens and toxins in food. The biosensor operates by sensing direct binding without the requirement for secondary fluorescent reporter molecules, or other intermediate chemical reactions. The results of analytical and simulation analysis of the performance of the biosensor has shown its potential for real-time detection and screening with significantly improved detection sensitivity. The biosensor can be configured as a portable "calculator-sized" unit with simple operating characteristics. Because of its potential portability, without reliance on supplies the fieldable units would be used in state and local water quality contaminant detection for both testing, process control and event detection. These uses provide a significant market. PROPOSED COMMERCIAL APPLICATION: We expect that a "real-time" portable multi-pathogen detection system for direct use in large scale food processing will provide the mechanisms required by the industry to maintain high efficiency at reduced manpower and supply costs. At the same time the turn around time will significantly reduce potential liability attached to contamination events. Other commercial applications include water quality, pathogen contamination detection for mandated municipal and state water quality programs.