QUANTITATIVE DETECTION OF CYTOMEGALOVIRUS BY USING A COM

巨细胞病毒（CMV）可能是破坏性的病人谁经历了异基因骨髓移植。在这些患者中快速检测CMV感染可以挽救生命，允许及时给予抗CMV药物。临床上还需要对临床标本中存在的CMV进行定量测量，以监测治疗并预测耐药变体的出现。一家商业供应商开发了一种研究测定法，允许使用定量聚合酶链反应（PCR）来测量从全血中提取的CMV DNA。我们正在使用由美国医学实验室收集的外周血白细胞评价这种PCR检测，并将结果与CMV抗原血症定量检测（在美国医学实验室进行）的结果进行比较，该检测检测多形核白细胞和单核细胞中CMV pp65早期结构蛋白的存在。结果不一致的标本将被送至微生物学参考实验室进行第二次PCR检测。迄今为止的结果表明，商业PCR至少与CMV抗原血症测定一样敏感。需要测试更多的样本以进行统计学上有效的比较。该项目已停止。

Cytomegalovirus (CMV) can be devastating in the patient who has undergone an allogenic bone marrow transplant. Rapid detection of CMV infection in these patients can be lifesaving, allowing the prompt administration of anti-CMV drugs. There is also a clinical need for a quantitative measure of the CMV present in the clinical specimen to allow for monitoring therapy and predicting emergence of drug-resistant variants. A research assay has been developed by a commercial vendor that allows the use of quantitative polymerase chain reaction (PCR) to measure the CMV DNA extracted from whole blood. We are evaluating this PCR assay using peripheral blood leukocytes collected by American Medical Laboratories and comparing the results with those of the CMV antigenemia quantitative assay (performed at American Medical Laboratories) that detects the presence of CMV pp65 early structural protein in polymorphonuclear leukocytes and monocytes. Specimens with discrepant results will be sent to Microbiology Reference Laboratories to be evaluated using a second PCR assay. Results thus far indicate that the commercial PCR is at least as sensitive as the CMV antigenemia assay. More specimens need to be tested for a statistically valid comparison to be made.This project has been discontinued.