A DeltaVision Restoration Microscope forCNR Imaging

描述(由申请人提供)：本建议的目的是获得 NIH为CNR生物的DeltaVision修复显微镜提供资金 成像设备。该显微镜系统将加大科研和培训力度 生物成像设施的能力，具体地说，将允许 本提案中列出的七大用户的研究目标 往前走。生物成像设备是唯一的光学显微镜成像设备 校园里的实验室，因此是生物学研究的起点 加州大学伯克利分校的许多研究人员使用了光学显微镜。一个 然而，主要的限制是无法分辨微弱的荧光，或者 分辨率有限的样本。所要求的仪器将填补急需的 自然资源与科学技术学院光学仪器的差距 加州大学伯克利分校的普通科学界。 在过去的一年里，我们对七种生物进行了初步实验 系统，比较在DeltaVision上使用所获得的结果 使用成像设备的蔡司获得的反卷积显微镜 510共聚焦显微镜。对于每个项目，我们提供的证据表明 DeltaVision修复显微镜远远超过蔡司的能力 510的分辨率和灵敏度。作为一个群体，研究人员在 这一建议涉及微生物和亚微生物的许多方面 生物事件；所有这些事件都需要去卷积显微镜来充分 想象他们特定的生物问题。这篇文章中描述的研究 建议涉及荧光可视化：(1)调节蛋白 枯草芽孢杆菌中的SpoIlR、SpoIIGA和Pro-I，(2)GFP标记 AcMNPV病毒粒子感染昆虫细胞(Volkman)，(3)亚细胞 VIRB蛋白的定位和T-转运蛋白的共定位 农杆菌(Zambryski)中的成分，(4)线粒体结构变化 在成纤维细胞老化过程中，(5)转录延伸因子Sdtlp 在酵母(凯恩)中，(6)剂量补偿和SMC蛋白的3D共定位 秀丽线虫粗线染色体(Meyer)和(7) 转录因子在间期的特性和共定位 果蝇黑腹细胞(TJAN)。

DESCRIPTION (provided by applicant): The purpose of this proposal is to acquire NIH funding for a DeltaVision Restoration microscope for the CNR Biological Imaging Facility. This microscope system will augment the research and training capabilities of the Biological Imaging Facility, and specifically, will permit the research goals of the seven Major Users listed in this proposal to proceed forward. The Biological Imaging Facility is the only optical microscope imaging laboratory on campus, and as such is the starting point for biological research involving optical microscopy for a great many researchers at UC Berkeley. A major limitation, however, is the inability to resolve dim fluorescence, or resolution-limited samples. The requested instrument will fill a much-needed gap in optical instrumentation in the College of Natural Resources and the general scientific community at the University of California, Berkeley. Over the last year we performed preliminary experiments on seven biological systems, comparing the results obtainable from use on a DeltaVision deconvolution microscope to that obtained using the Imaging Facility's Zeiss 510 confocal microscope. For each project we present evidence showing that the DeltaVision restoration microscope far surpasses the capabilities of the Zeiss 510 in resolution and sensitivity. As a group, the researchers described in this proposal are involved in myriad aspects of microbial and sub-microbial biological events; all of which require deconvolution microscopy to adequately visualize their particular biological questions. The research described in this proposal involves fluorescence visualization of: (1) regulatory proteins SpoIlR, SpoIIGA, and pro-i in Bacillus subtilis (Hofmeister), (2) GFP-Iabeled AcMNPV virions during infection of insect cell (Volkman), (3) the suboellular localization of VirB proteins and the colocalization of T-Transporter components in Agrobacterium (Zambryski), (4) mitochondria structure changes during aging in fibroblasts (Ames), (5) transcription elongation factors Sdtlp in yeast (Kane), (6) 3D colocalization of dosage-compensation and SMC proteins on the Caenorhabditis elegans pachytene chromosomes (Meyer), and (7) characterization and colocalization of transcription factors in interphase Drosophila melanogaster cells (Tjian).