Myenteric Neuroplasticity Due to Experimental Colitis

描述（由申请人提供）：存在的电机干扰 在肠道炎症期间，反映了 炎症介质和肠神经系统。的神经机制 炎症诱导的运动障碍目前是未知的。用豚鼠 实验性结肠炎模型，本提案的具体目的是测试 两个假设。第一个假设是炎症引起了 内在初级传入神经元的兴奋性，导致不平衡 在蠕动反射回路中。这一假设将由 解决两个问题：1）电或突触特性是否 功能鉴定的肌间神经元在实验性结肠炎中的变化？ 2)是豚鼠结肠的神经原性推进运动 在炎症过程中发生了变化第二个假设是， 蠕动反射回路，由炎症引起，持续以下 组织病理学恢复。为了验证这一假设，将提出两个问题： 解决：1）功能上的电或突触特性的变化 鉴定的肌间神经元在组织学恢复后持续存在或出现 2）神经原性推进运动活动的变化， 完整的结肠持续存在或从炎症中恢复后出现？在 在进行这些研究时，我们会把 技术，包括神经元的细胞内记录和标记， 逆行轴突追踪、免疫组织化学和运动性测定。这些 结果将促进我们对神经免疫机制的理解， 与炎症性肠相关的整合和动力障碍 疾病和功能性肠病。

DESCRIPTION (provided by applicant): The motor disturbances that are present during inflammation of the bowel reflect a significant interaction between inflammatory mediators and the enteric nervous system. The neural mechanisms of inflammation-induced dysmotility are currently unknown. Using a guinea pig model of experimental colitis, the specific aims of this proposal are to test two hypotheses. The first hypothesis is that inflammation causes an increased excitability in intrinsic primary afferent neurons, which leads to an imbalance in the peristaltic reflex circuit. This hypothesis will be explored by addressing two questions: 1) Do the electrical or synaptic properties of functionally identified myenteric neurons change during experimental colitis? 2) Is the neurogenic propulsive motor activity of the intact guinea pig colon altered during inflammation? The second hypothesis is that changes in the peristaltic reflex circuit, induced by inflammation, persist following histopathological recovery. To test this hypothesis two question will be addressed: 1) Do changes in the electrical or synaptic properties functionally identified myenteric neurons persist or arise following histological recovery from inflammation: 2) Do changes to the neurogenic propulsive motor activity of the intact colon persist or arise following recovery from inflammation? In conducting these studies, we will implement integrated combinations of techniques, including intracellular recording and labeling of neurons, retrograde axonal tracing, immunohistochemistry, and motility assays. These results will advance our understanding of the mechanisms of neuro-immune integration and motility disturbances associated with inflammatory bowel disease and functional bowel disorders.