CHARACTERIZATION OF ESTROGEN BINDING PROTEIN FROM CANDIDA ALBICANS

我们正在继续研究雌激素结合蛋白，从 白色念珠菌在体内建立这种氧化还原功能 酵素。作为一种方法，我们正在研究突变体的性质 活性中心残基用于识别那些催化和/或改变的 在体内捕获可能有用的配体结合特性 底物。计算机图形学实验室在帮助 US以可视化并基于以下内容生成酶的同源模型 酵母菌高度同源酶的结构研究 以产生感兴趣的突变体。此外, 我们正在使用图形来尝试解释观察到的差异 突变型和野生型酶的行为，例如，pH依赖性 行为，在活性部位附近残基的背景下。

We are continuing studies of the estrogen binding protein from Candida albicans to establish the in vivo function of this redox enzyme. As one approach we are studying the properties of mutants of active site residues to identify those with altered catalytic and/or ligand binding properties that may be useful in trapping in vivo substrates. The Computer Graphics Laboratory is critical in helping us to visualize and generate a homology model of the enzyme based on the structure of a highly homologous enzyme from Saccharomyces cerevisiae in order to generate the mutants of interest. In addition, we are using the graphics to try to interpret observed differences in the behavior of the mutant and wild type enzymes, e.g., pH dependent behavior, in the context of residues near the active site.