XRAY DIFFRACTION ANALYSES OF PLANT VIRUSES

我们已经使用同步辐射进行高分辨率结构 植物病毒的研究 衍射数据收集自 卫星黍花叶病毒（SPMV）的晶体，导致1.9 E T=1病毒的解析结构。 晶体衍射数据 芜菁黄花叶病毒（TYMV），一种T=3病毒，具有非常大的 单位细胞，也收集到3.5 E分辨率。 这些数据， 使用传统的X射线发生器是无法实现的， TYMV的结构测定。 植物病毒结构 研究对于理解病毒结构很重要，但也 提供了一种方便的方法来研究X射线数据 可以完成大单位单元的收集和数据处理 成功地

We have used synchrotron radiation for high resolution structural studies of plant viruses. Diffraction data were collected from crystals of Satellite Panicum Mosaic Virus (SPMV) leading to a 1.9 E resolution structure of the T=1 virus. Diffraction data from crystals of Turnip Yellow Mosaic Virus (TYMV), a T=3 virus with a very large unit cell, were also collected to 3.5 E resolution. These data, which are unattainable using conventional X-ray generators, are being used in the structural determination of TYMV. Plant virus structural studies are important in understanding virus structure, but also provide a convenient way to study the ways in which X-ray data collection and data processing of large unit cells can be done successfully.