CORE--NMR

近年来，多维NMR光谱学的进展已经取得了进展， 蛋白质三维结构的测定 (of大小小于25 kD）和肽。此外，边- 链和主链动力学测量允许确定位点- 关于灵活性区域的具体信息。的内部运动 蛋白质，例如单个氨基酸残基的旋转以及 残基群的协同节段运动，广泛发生在 不同的时间尺度和随机热运动的结果，以及 与特定配体和/或蛋白质的相互作用。这些波动 被认为是非常重要的生物功能，包括 酶催化，变构，蛋白质-蛋白质相互作用和配体 约束力目前对这些运动细节的了解是零碎的。 核心主任已经使用这些方法来研究折叠和动力学 抹香鲸肌红蛋白此外，主任还雇用了 用于探测蛋白质-配体结合的异源过滤方法 交互.她将继续使用这些方法来调查 蛋白质动力学在具有生物学重要性的蛋白质系统中的作用。我们 已经组装了一个核心设施， 项目的所有研究者。 1)提供蛋白质核磁共振测量方面的专业技术。 2)为设计新的实验提供帮助， 具有生物学意义的问题 3)为研究人员提供NMR光谱学使用方面的培训。 核心设施的组织是为了提供最大的合作潜力 为所有研究人员，并最大限度地利用最新的国家- 艺术技术来解决生物学上的重要问题。

In recent years, advances in multi-dimensional NMR spectroscopy have made possible the determination of the three-dimensional structures of proteins (of sizes less than 25 kD) and peptides in solution. In addition, side- chain and backbone dynamics measurements allow the determination of site- specific information on regions of flexibility. Internal motions of proteins, such as rotations of individual amino acid residues as well as concerted segmental motions of groups of residues, occur on widely different time scales and result from random thermal motions as well as interactions with specific ligands and or proteins. These fluctuations are recognized as being very important in biological functions, including enzyme catalysis, allosterism, protein-protein interactions and ligand binding. Current knowledge of the details of these motions is fragmentary. The Core Director has used these methods to study the folding and dynamics of sperm whale myoglobin. In addition, the Director has employed heteronuclear filtering methods to probe protein-ligand binding interactions. She will continue to use these methods to investigate the role of protein dynamics in protein systems of biological importance. We have assembled a core facility which will give access to this technology to all the Investigators of the Program Project. 1) to provide technical expertise in protein NMR measurements. 2) to provide aid in the design of new experiments to target specific questions of biological importance 3) to provide training to investigators in the use of NMR spectroscopy. The core facility is organized to offer maximum collaborative potential for all investigators, and to maximize the use of the latest state-of-the- art techniques to address problems of biological importance.