LIPID & PROTEIN TRAFFIC & DYNAMICS IN EPITHELIAL CELLS

上皮细胞，占身体所有细胞的50%[和 占所有人类癌症的90%]具有定义功能， 创造和维持身体的生理区室。 上皮细胞不仅在物理上分隔不同的环境; 它们调节它们之间的选择性交换。 这就要求 细胞的相对表面是功能性分化的;因此， 上皮细胞在功能上是极化的。 这种两极分化是 通过合作组建和动态维护， 相邻细胞之间的连接复合物，并通过选择性或 膜蛋白和脂质的差异递送和保留 每一个细胞的一极或另一极。 该计划中的项目 研究将组分选择性递送至顶侧和基底侧 膜结构域 采取了各种不同的观点， 技术的使用，但同时有一个共同的重点， 所有四个项目的高尔基复合体，考虑到其横向 组织和异构性、流量、组成和完整性 并保持其空间方向。 Edidin（项目1） 着重于高尔基体膜和细胞质的横向组织 膜蛋白，特别是GPI蛋白和脂质， 这些分子的极化运输。 哈伯德（项目3） 重点是细胞内的路线和机制， 传递和保留膜蛋白，重点是 内体/转胞吞系统。 Machamer（项目4）侧重于 高尔基复合体的组织和组成机制 对脂质和蛋白质的分类很重要， 细胞膜，同时保持自身的完整性。 Schroer （项目6）的重点是维持细胞骨架机制， 上皮细胞的极化细胞内结构， 囊泡在细胞质膜之间和细胞膜之间的 细胞质和表面。

Epithelial cells, which comprise 50% of all cells in the body [and account for 90% of all human cancers] have as a defining function the creation and maintenance of physiological compartments of the body. Epithelial cells not only physically separate different environments; they regulate selective exchange between them. This requires that the cells' opposing surfaces be functionally differentiated; hence, epithelial cells are functionally polarized. This polarization is accomplished through cooperative formation and dynamic maintenance of junctional complexes between adjacent cells and by the selective or differential delivery and retention of membrane proteins and lipids to one pole or the other each cell. The projects in this program study selective delivery of components to apical and basolateral membrane domains. A variety of perspectives are taken and different techniques are used, but at the same time there is a common focus in all four projects on the Golgi complex, considering its lateral organization and heterogeneity, traffic, composition, and integrity and maintenance of its spatial orientation. Edidin (Project 1) focuses on the lateral organization of Golgi membrane and plasma membrane proteins, particularly GPI-proteins, and lipids, required for the polarized traffic of these molecules. Hubbard (Project 3) focuses on intracellular routes and mechanisms for the polarized delivery and retention of membrane proteins, with emphasis on the endosomal/transcytotic system. Machamer (Project 4) focuses on the mechanisms of the organization and composition of the Golgi complex that important for sorting lipids and proteins destined for the plasma membrane, while maintaining its own integrity. Schroer (Project 6) focuses on the cytoskeletal machinery that maintains the polarized intracellular architecture of epithelial cells and on the routes traveled by vesicles between cytoplasmic membranes and between cytoplasm and surface.