REPBASE UPDATE- A DATABASE OF REPETITIVE SEQUENCES

Repbase Update （http://www.girinst.org）是一个全面的DNA序列集合，目前代表了来自所有真核生物物种的重复元件的1200多个家族和亚家族。每个家庭都有一个独特的名字，参考已发表的文献，并经常在简短的评论中描述。在过去的两年中，数百个重复家族被添加到Repbase Update中，这些重复家族要么在其他地方没有被报道过，要么在它们的一致序列和生物学分类方面被彻底修改过。我们的方法是基于计算机辅助探索和分析公开可用的DNA序列数据。Repbase Update已被世界各地的公共机构和私营公司常规用于基因发现、序列组装和探针设计。此外，Repbase Update成为生物和医学重要性的个人研究项目的独特资源。鉴于预计会有大量新的序列数据，我们建议继续开发、维护和电子传播这一资源。特别是，我们希望发现和描述数百个新的重复家族，这些家族将继续以电子方式在Repbase Update中发布。此外，我们将继续编译和组织所有真核生物物种的重复序列。最后，我们将维护公共服务器，用于人类和啮齿动物重复序列的自动注释，并生成哺乳动物基因组DNA中重复元素的最新注释。我们特别建议：1。继续开发、维护和电子分发所有已测序真核物种的重复元素参考集合。2. 生成重复元素的公共领域地图存在于可用的哺乳动物基因组DNA序列。

Repbase Update (http://www.girinst.org), is a comprehensive collection of DNA sequences, currently representing over 1200 families and subfamilies of repetitive elements from all eukaryotic species. Each family is identifiable by a unique name, referenced to published literature and often characterized in brief commentaries. Several hundreds of repetitive families added to Repbase Update during the last two years are either unreported anywhere else, or have been thoroughly revised in terms of their consensus sequences and biological classification. Our approach is based on computer-assisted exploration and analysis of publicly available DNA sequence data. Repbase Update has been routinely used by public institutions and private companies throughout the world in routine gene discovery, sequence assembly and probe design. In addition, Repbase Update became a unique resource for individual research projects of biological and medical importance. In light of the anticipated flood of new sequence data, we propose continuation of development, maintenance and electronic dissemination of this resource. In particular, we expect to discover and characterize hundreds of new repetitive families which will continue to be published electronically in Repbase Update. Furthermore, we will continue to compile and organize reported families of repeats from all eukaryotic species. Finally, we will maintain the public server for automatic annotation of human and rodent repeats and generate up-to-date annotation of repetitive elements present in genomic DNA of mammals. In particular we propose to: 1. Continue development, maintenance and electronic distribution of reference collections of repetitive elements from all sequenced eukaryotic species. 2. Generate public domain maps of repetitive elements present in available mammalian genomic DNA sequences.