CORE--FLOW CYTOMETRY FACILITY

研究人员在该中心的所有项目中大量使用CFAR流式细胞术。核心为CFAR成员提供关于这种强大的分析方法的潜在用途的教育和咨询，以及适当的实验设计。除了通常的淋巴细胞三色表面表型，CORE还提供了一系列创新的测量方法。我们的仪器包括两个FACSCAN和两个Profile分析流式细胞仪，每个都配备了三色检测，一个Becton Dickinson(BD)FACSCalibur和一个B.D.Vantage双激光分选仪和克隆沉积设备。本仪器位于负压室内，对所有离开仪器的空气进行BL-3类空气过滤和HEPA过滤。这使我们能够对受感染的细胞进行分类。向使用该设施的科学家收取费用可部分收回成本。

The CFAR Flow Cytometry is heavily used by investigators in all programs of the Center. The core provides education and consultations with CFAR members concerning the potential uses of this powerful method of analysis, and the appropriate design of experiments. Core provides an array of innovative measurements in addition to the usual 3- color surface phenotyping of lymphocytes. Our instrumentation consists of 2 FACSCAN and 2 Profile analytical flow cytometers, each equipped for three-color detection, a Becton Dickinson (BD) FACSCalibur and a B.D. Vantage dual laser sorting instrument with a cloning deposition device. This instrument is located in a negative air pressure room with BL-3 category air filtration and HEPA filtration of all air leaving the instrument. This enable us to sort infected cells. Charge back fees to scientists using the facility provide partial recovery of costs.