CORE--ANALYTICAL FACILITY /LIPIDS

描述(改编自应用程序)：当临床营养 研究单位(CNRU)于1979年首次获得资助，是唯一的核心设施 管理/临床核心是分析核心。初级阶段 这个核心的功能是开发和验证维生素微量矿物质分析。 对于CNRU的调查人员来说。随着拨款在1984年的延续， 分析核心扩大到包括氨基酸分析和化验 对于几种血液蛋白，包括视黄醇结合蛋白，转铁蛋白， 前白蛋白和白蛋白。此外，在此期间，实验室 开发了更灵敏的维生素高效液相色谱分析方法，包括 维生素A和E，以及维生素B2和B6。新的 还增加了用于氨基酸定量测定的仪器。在……里面 此外，由于人们对涉及血脂和 脂质过氧化，我们开始扩大实验室的进程，以包括 对组织、细胞和血液中脂肪成分的定量测量。 研究脂质过氧化损伤的新方法也被添加到 核心提供的服务。对涉及以下方面的调查的需求日益增长 氨基酸代谢促进了核心实验室的更强有力的合作 与外科、分子生理学和生物物理学系，以及 医学，特别是糖尿病研究和培训中心(DRTC)。至 为了满足所有需求，分析核心再次进行了重组， 病理系拉里·L·斯威夫特博士被任命为主任。 核心被细分为三个专业领域：氨基酸部分， 脂质/脂质过氧化部分和维生素/微量元素部分， 与每个部分直接独立。

DESCRIPTION (adapted from the application): When the Clinical Nutrition Research Unit (CNRU) was first funded in 1979, the only core facility other than the Administrative/Clinical Core was the Analytical Core. The primary function of this core was to develop and validate vitamin-trace mineral assays for the CNRU investigators. With the continuation of the grant in 1984, the Analytical Core was expanded to include amino acid analyses as well as assays for several blood proteins, including retinol binding protein, transferrin, prealbumin, and albumin. In addition, during this period the laboratory developed more sensitive HPLC assays for vitamins, including assays for vitamins A and E, as well as for the vitamers of vitamins B2 and B6. New instrumentation was also added for quantitative amino acid determinations. In addition, because of an increased interest in research involving lipids and lipid peroxidation, we began the process of expanding the laboratory to include quantitative measurements of the lipid components of tissue, cells, and blood. New methods for studying lipid peroxidative injury were also added to the services provided by the core. A growing need for investigations involving amino acid metabolism prompted a stronger collaboration of the Core Laboratory with the Departments of Surgery, Molecular Physiology and Biophysics, and Medicine, in particular the Diabetes Research and Training Center (DRTC). To accommodate all of the needs, the Analytical Core was reorganized once again, and Dr. Larry L. Swift, Department of Pathology, was appointed the Director. The core was subdivided into three areas of specialization: Amino Acid Section, The Lipids/Lipid Peroxidation Section, and the Vitamin/Trace Element Section, with each section directly independently.