ABNORMAL WATER METABOL IN TYPE 1A ANGIOTENSIN RECEPTOR DEFICIENT MICE: MUTANT

本课题的研究目标是磁共振成像与光学组织学的相关性 AET诱导的脑损伤模型。 最近的结果 MRM显示，在脑标本中检测到神经元损伤， 用DOM处理的动物。 在MRM中看到的一些病变 在常规光学切片中，H&E样本不明显 和尼氏染色。 如果这些结果可以在其他模型中得到证明 疾病的影响是巨大的。 简单地说，这表明 MRM可以检测到传统组织学中看不到的病变。 这 与中心最近的其他证据一致 共9 将扫描样本。 这些是老鼠大脑的一半， 用AET治疗。 另一半大脑已经 切片并用常规组织学分析。 其中六 标本在四个不同的核中显示出明显的损伤：上级核 橄榄核、杏仁核、红核、面神经核。 损害范围 从10%的细胞核到97%的细胞核 我们将 用2种方案扫描第二个1/2脑：IFSE-TR = 1.5秒; TE=50 ms; Nechos=8; fovz=fovy=10 mm; fovx=20 mm;矩阵（z，y，x）= 256 x 256 x 128; nex=2（总时间6.8小时）II GRASS TR=100 ms.TE=3 ms，alpha=350， 与I相同的fov矩阵，nex=4（总时间=3.64小时）我们将对 MR后的标本，并将特定的感兴趣区域与 定量形态测量以描述%细胞损伤。

The goal of this project is correlative MR and optical histology of a model of brain damage induced by AET. Recent results have demonstrated neuronal damage detected by MRM in brain specimens from animals treated with DOM. Some of the lesions seen in the MRM specimens were not evident in conventional optical sections with H&E and Nissl stain. If these results can be demonstrated in other models of disease, the implications are enormous. Simply put, this suggests that MRM can detect lesions not seen in conventional histology. This is consistent with other recent evidence in the Center. A total of 9 specimens will be scanned. These are 1/2 the brains of rats that have been treated with AET. The other half brains have already been sectioned and analyzed with conventional histology. Six of these specimens show significant damage in four different nuclei: Superior Olive, trapezial nucleus, red nucleus, facial nucleus. Damage ranges from 10% of the nucleus to as much as 97% of the nucleus. We will scan the second 1/2 brain with 2 protocols: I FSE -TR=1.5sec; TE=50 ms; Nechos=8; fovz=fovy=10 mm; fovx=20 mm; matrix (z,y,x)=256x256x128; nex=2 (Total time 6.8 hrs) II GRASS TR=100 ms.TE=3 ms, alpha=350 , same fov matrix as I, nex=4 (total time =3.64 hrs) We will section the specimens after MR and compare specific regions of interest with quantitative morphometry to describe % cell damage.