MICHIGAN NIDDK BIOTECHNOLOGY CENTER

这项提案的总体目标是在密歇根大学建立一个NIDDK生物技术中心。该中心将为测量基因表达的广泛模式提供最先进的技术、设备和建议。该中心将向由NIDDK资助的调查人员或从事与NIDDK任务明确相关的研究人员开放。这项申请将专注于DNA微阵列技术，这一方法非常适合密歇根大学现有的优势、资源和承诺。与这一应用相关的资源包括物理空间，其中包括用于存储和复制EST的适当设备、用于生产和杂交微阵列芯片的设备以及用于数据分析的工具。人员资源包括受过培训的执行上述步骤的工作人员，包括准备标记的探针和在RNA制备和数据分析方面向调查人员提供指导和咨询的专家。除了这种个性化的教学，NIDDK生物技术中心还将通过一系列研讨会向更广泛的社区提供教育。该中心将由一个内部咨询委员会管理，该委员会包括来自NIDDK资助的三个现有中心(糖尿病研究和培训中心、肾脏中心和肠道/‘肽中心)的代表，这些中心的成员需要获得用于表达分析的技术。这三个中心将为NIDDK生物技术中心和医学院提供补充资金支持。内部咨询委员会将监督该中心的所有方面，并将审查个别调查人员提交的获取微阵列资源的申请。两名在微阵列技术前沿具有全国声誉的外部顾问将担任顾问。这项建议将受益于出色的大学支持--大学已承诺提供近200万美元，用于购买和分发所有可用的人类、大鼠和小鼠EST(序列验证)，这些EST将在一个中央设施复制并分发给包括NIDDK生物技术中心在内的许多有针对性的研究项目。这些研究计划分享微阵列技术的知识和经验，并开发创新的应用，包括基于寡核苷酸的新型阵列和将微阵列分析与蛋白质组分析相结合的方法。因此，肥沃的环境将确保NIDDK生物技术中心有效地公布与表达分析相关的进展。

The overall goal of this proposal is to establish an NIDDK Biotechnology Center at the University of Michigan. The Center will provide access to state-of-the-art technology, equipment and advice for measuring broad patterns of gene expression. The Center will be accessible to investigators funded by the NIDDK or doing research clearly related to the mission of the NIDDK. This application will focus on DNA microarray technology, an approach that fits well with the existing strengths, resources and commitments of the University of Michigan. Resources connected with this application consist of physical space containing appropriate equipment for storing and replicating ESTs, equipment for producing and hybridizing microarray chips, and tools for data analysis. Personnel resources include trained staff for performing the above steps, including preparation of labeled probes and experts to provide guidance and advice to investigators in terms of RNA preparation and data analysis. In addition to such individualized instruction, the NIDDK Biotechnology Center will provide education to the broader community through a series of seminars. The Center will be governed by an Internal Advisory Committee that includes representatives from three existing NIDDK-funded Centers (Diabetes Research and Training Center, Renal Center, and Gut/'Peptide Center) whose members have needs for access to technologies for expression analysis. These three Centers will provide supplemental financial support for the NIDDK Biotechnology Center, as will the Medical School. The Internal Advisory Committee will oversee all aspects of the Center and will review applications submitted by individual investigators for access to microarray resources. Two external advisors with national reputations at the forefront of microarray technology will serve as consultants. This proposal will benefit from outstanding University support - the University has committed close to $2 million to acquire and distribute all sets of human, rat and mouse ESTs (sequence verified) available which will be replicated and distributed at a central facility to a number of targeted research programs including the NIDDK Biotechnology Center. These research programs share knowledge and experience in microarray technologies and develop innovative applications including novel oligonucleotide based arraying and means to integrate microarray analysis with proteomic analysis. Thus a fertile environment will ensure that advances made that are relevant to expression analysis will be promulgated efficiently by the NIDDK Biotechnology Center.