Core--Tissue culture

描述：该核心由一个设备组成，该设备可协助所有参与实验室进行组织培养实验，并提供组织学实验协助。组织培养将用于该计划的所有四个组成部分，通过细胞转染试验和/或神经突生长、突触发生和脊柱形成试验来分析细胞粘附分子（Ranscht）、受体蛋白酪氨酸激酶（Pasquale）和细胞表面蛋白聚糖（Stallcup和Yamaguchi）的作用和机制。组织培养将在细胞系和原代神经培养物中转染cDNA构建物，并建立海马原代培养物以测定神经元分化和突触发生，从而协助本项目的研究。这个核心的一个主要优点是海马体培养系统是标准化的，因此所研究的一个分子的结果可以与其他分子的结果相关联。这将扩大对神经元分化和突触发生的分子本质的理解，并促进项目实验室之间的额外互动和联合实验。该中心还将通过切割冷冻切片和石蜡切片来协助组织学实验，并用各种抗体进行免疫染色。

Description: This core consists of a facility that assists all participating laboratories with tissue culture experiments, and provides assistance with histological experiments. Tissue culture will be used in all four components of this program to analyze the role and mechanism of cell adhesion molecules (Ranscht), receptor protein tyrosine kinases (Pasquale) and cell surface proteoglycans (Stallcup and Yamaguchi) by cell transfection assays and/or assays for neurite growth, synaptogenesis, and spine formation. The Tissue Culture will assist investigations of this Program Project with the transfection of cDNA constructs in cell lines and primary neural cultures, and setup primary cultures from the hippocampus to assay neuronal differentiation and synaptogenesis. One major advantage of this core is that the hippocampal culture system is standardized, so that results obtained for one of the studied molecules can be related to those for others. This will broaden the understanding of the molecular nature of neuronal differentiation and synaptogenesis, and foster additional interactions and joint experiments between the program project laboratories. The Core will also provide assistance with histological experiments by cutting frozen and paraffin sections, and perform immunostaining with various antibodies.