Core--Mass spectrometry

这个质谱核心的广泛目标是提供高端质谱技术，以支持这个超级基金基础研究计划中的生物医学和非生物医学项目，并开发和应用一种新的，高度特异性和灵敏度的质谱技术，以检测和量化环境化合物及其代谢物。这些化合物包括四氯乙烯、1，1，2-三氯乙烯、顺式-1，2-二氯乙烯、氯乙烯、氯乙醛和氯仿。这种新仪器的发展将需要一个气相色谱电子单色谱飞行时间质谱仪（GC-EM-TOF-MS）的建设，以分析负离子使用EM的电离电子的单能束的生产结合脉冲提取。为了支持该应用中的其他生物医学和非生物医学项目，我们将开发和应用质谱方法来分析蛋白质和烃溶剂代谢产物之间形成的加合物。我们将开发质谱分析方法，用于分析与2，5-己二酮及其类似物3-甲基-2，5-己二酮、3，4-二甲基-2，5-己二酮、3-4-二乙基-2，5-己二酮、2，9-二甲基-4，7-癸二酮和1，2-二乙酰基苯反应产生的蛋白质和肽。γ-二酮烃加合物对神经丝蛋白质结构的热稳定性的影响将通过氢-氘交换（H/D）交换和平衡热变性研究结合电喷雾质谱来评估。碳氢化合物加合物对神经丝蛋白亚基构象的影响将通过动力学H/D交换实验以及电喷雾质谱法来确定。

The broad objective of this Mass Spectrometry Core is to provide high- end mass spectroscopy techniques to support the biomedical and non- biomedical projects in this Superfund Basic Research Program and to develop and apply a new, highly specific and sensitive mass spectroscopy technique to detect and quantify environmental compounds and their metabolites. These compounds will include tetrachloroethene, 1,1,2- trichloroethene, cis-1,2-dichloroethene, vinyl chloride, chloroacetaldehyde and chloroform. The development of this new instrument will require the construction of a gas chromatograph electron monochromater-time-of flight-mass spectrometer (GC-EM-TOF-MS) to analyze negative ions using the EM for the production of monoenergetic beams of ionizing electrons in combination with pulsed extraction. To support the other biomedical and non-biomedical projects in this application, we will develop and apply mass spectrometric methods to analyze adducts formed between proteins and hydrocarbon solvent metabolites. We will develop mass spectrometric methods for analyzing protein and peptides resulting from reaction with 2,5-hexanedione, and analogs, 3-methyl-2,5-hexanedione, 3,4-dimethyl-2,5-hexanedione, 3-4- diethyl-2,5-hexanedione, 2,9-dimethyl-4,7, decanedione and 1,2- diacetylbenzene. The effects of gamma-diketone hydrocarbon adducts on the thermal stability of neurofilament protein structures will be a assessed by hydrogen-deuterium exchange (H/D) exchange and equilibrium thermal denaturation studies in conjunction with electrospray mass spectroscopy. The effects of hydrocarbon adducts on the conformation of neurofilament protein subunits will be determined by kinetic H/D exchange experiments, together with electrospray mas spectrometry.