Measurement Of Cytokines In Cervical Fluids

疱疹病毒和肿瘤之间的关系已经有了很好的文献记载，尽管宿主因素在这种关系中所起的作用仍不清楚。该项目的主要目标是确定从感染肿瘤类型的疱疹乳头瘤病毒(HPV)的妇女宫颈标本中测量的多分析细胞因子谱是否可以预测随后进展为高度宫颈上皮内瘤变(CIN)的风险。使用一种名为循环免疫亲和层析的技术，将对宫颈分泌液中的细胞因子谱进行检查，以确定在肿瘤过程中哪些细胞因子和其他因素似乎升高或激活。30个分析小组将被用来比较高度CIN女性样本与致瘤性HPV感染女性样本和正常对照组样本中的细胞因子谱。从这样的研究中，人们希望确定哪些细胞因子或其他因素是预测肿瘤进展的候选因素。一旦确定了这一点，这些标记将用于对HPV和CIN自然历史的前瞻性研究。

The relationship between Herpes viruses and neoplasia has been quite well documented although the role played by host factors in this relationship is still unknown. The prime objective of this project to determine whether multi-analyte cytokine profiles measured in cervical specimens, from women infected with oncological types of Herpes Papilloma Virus (HPV), can predict risk of subsequent progression to high-grade cervical intraepithelial neoplasia (CIN). Using a technique called recycling immunoaffinity chromatography, examination of cytokine profiles in cervical fluid will be performed to determine which cytokines and other factors appear to elevated or activated during the neoplastic process. 30 analyte panels will be employed to compare cytokine profiles in samples from women with high-grade CIN to samples from women with oncogenic HPV infection and normal controls. From such studies it is hoped to determine which cytokines or other factors are candidates for predicting neoplastic progression. Once this has been established these markers will be used in a prospective study of HPV and CIN natural history.