TEMPLATE POLYMERIZATION

分子印迹是在交联网络聚合物中为有机分子建立结合位点和受体位点的一种常用方法。本提案的目标是扩展分子印迹的边界，以创建药物和药物中间体、寡核苷酸和其他复杂有机靶点的人工受体和结合位点。具体来说，我们建议通过分子印迹技术来扩大分子的靶向范围。开发含全氟烷基的酸性化合物和分子印迹新配方。我们还建议在药物合成中合成苯二氮卓类药物、寡核苷酸、碳水化合物衍生物、小肽和中间体的受体。将印迹聚合物集成到有用的技术中需要能够以各种形态制造这些材料。沿着这些路线，我们已经开发了分子印迹薄膜和膜（MIM’s）的方法。我们建议对这些材料的许多应用进行评估，包括它们在固相萃取装置中的聚合物相使用，以及作为分离复杂有机分子和对映体的半透膜。印迹薄膜也将被评估为传感器设备的识别元素。后一种应用将利用荧光信号来报告结合事件。最后，我们提出了一项研究，旨在了解在聚合过程中如何产生结合位点。

Molecular imprinting is a general method for creating binding and receptor sites for organic molecules in crosslinked network polymers. The objectives of this proposal are to expand the boundaries of molecular imprinting to create artificial receptors and binding sites for drugs and drug intermediates, oligonucleotides, and other complex organic targets. Specifically we propose to broaden the range of molecules that can be targeted by molecular imprinting. We will develop new formulations for imprinting acidic compounds and molecules that contain perfluoroalkyl groups. We also propose to synthesize receptors for benzodiazepines, oligonucleotides, carbohydrate derivatives, small peptides, and intermediates in drug synthesis. The integration of imprinted polymers into useful technology requires an ability to fabricate these materials in a variety of morphologies. Along these lines we have developed methods for molecularly imprinted thin films and membranes (MIM's). We propose to evaluate these materials for a number of applications, including their use as the polymer phase in solid phase extraction devices, and as semipermeable membranes for the separation of complex organic molecules and enantiomers. Imprinted films will also be evaluated as the recognition element for sensor devices. This latter application will utilize a fluorescent signal to report the binding event. Finally we have proposed a study designed to get at how binding sites are created during the polymerization process.