Metabonomic Urine Analysis: The INTERMAP Diet-BP Study

描述(由申请人提供)： InterMAP是一项基本的流行病学调查，旨在澄清有关饮食因素，特别是大量营养素(总、动植物蛋白、个别氨基酸、脂肪酸、胆固醇、糖)以及微量营养素在大多数中老年人普遍存在的不良血压模式的病因中所起的作用的未回答的问题。在NHLBI的支持下，关于饮食和血压的十个基本假设、四个亚组假设和多个探索性问题正在通过一项高质量的国际合作人口研究来解决，该研究涵盖了来自四个国家(中国、日本、英国、美国)的4,680名年龄在40-59岁之间的男性和女性-不同的民族-种族和社会人口背景-消费多样化的饮食。这些分析使用了所有4,680名参与者的营养摄入量的广泛数据，这些数据来自四次24小时召回和4个国家数据库(由INTERMAP开发)，涉及所有报告的食物的营养成分(包括71种营养素的数据)，以及两次24小时尿液收集(已分析)，涉及钠、钾、钙、镁、尿素、肌酐、体积和微量白蛋白尿。NHLBI资助的进一步研究正在进行中，包括分析每个人的尿氨基酸，以及对报告的膳食补充剂进行编码。这里提出的新工作具有新颖性、探索性、知识生成性和“竞争前”性质，与本RFA的目标一致。它涉及a)应用新的生化(代谢组学)技术，即高场核磁共振(核磁共振)光谱和高效液相色谱，以阐明所有INTERMAP尿样中的代谢模式和成分，以及b)分析这些尿代谢模式/成分与个人营养摄入量和血压的关系。它提供了一个独特的机会，可以在INTERMAP饮食回忆和尿液数据之间建立实质性的营养代谢BP联系，来自尿液核磁共振分析的多种代谢特征，以及任何其他研究在人群中没有的SBP/DBP联系的8个测量。核磁共振数据对于首次了解不同自由生活人群的尿液代谢排泄模式也是有意义的。这项研究涉及INTERMAP高级调查人员(芝加哥、伦敦)与伦敦帝国理工学院代谢组学研究中心的新合作，后者是生物体液高通量核磁共振测量的世界领先者。

DESCRIPTION (provided by applicant): INTERMAP is a basic epidemiologic investigation that aims to clarify unanswered questions on the role of dietary factors, particularly macronutrients (total, animal and vegetable protein, individual amino acids, fatty acids, cholesterol, sugars), also micronutrients, in the etiology of unfavorable blood pressure (BP) patterns prevailing for most middle-aged and older individuals. Supported by NHLBI, ten primary hypotheses, four subgroup hypotheses and multiple exploratory questions on diet and BP are being addressed by means of a high-quality international cooperative 17-sample population study of 4,680 men and women ages 40-59 from four countries (China, Japan, UK, US) - of varied ethnic-racial and socio-demographic backgrounds - consuming diverse diets. These analyses use extensive data on nutrient intake for all 4,680 participants, generated from four 24-hour recalls and the 4 country databases (developed by INTERMAP) on nutrient composition of all reported foods (with data on 71 nutrients), and from two 24-hr urine collections (already analyzed) on Na, K, Ca, Mg, urea, creatinine, volume and microalbuminuria. Further NHLBI-funded research in progress includes analysis of urinary amino acids of each individual, and coding of reported dietary supplements. New work proposed here is novel, exploratory, knowledge generating and "pre-competitive" in nature, in keeping with the aims of this RFA. It involves a) application of novel biochemical (metabonomic) techniques i.e., high field Nuclear Magnetic Resonance (NMR) spectroscopy and HPLC for elucidation of metabolic patterns and constituents in all INTERMAP urinary samples, and b) analyses of relations of these urinary metabolic patterns/constituents to nutrient intakes and BP of individuals. It offers a unique opportunity to make substantive nutritional-metabolic-BP links between INTERMAP dietary recall and urine data, multiple metabolic characteristics from urinary NMR analyses, and 8 measurements of SBP/DBP - links not available on populations from any other research. The NMR data are also meaningful "in their own right" to gain knowledge for the first time on urinary metabolic excretion patterns in diverse, free-living populations. The research involves new collaboration of senior INTERMAP investigators (Chicago, London) with the Center for Metabonomic Research, Imperial College, London, a world-leader in high-throughput NMR measurement of biofluids.