Detection of H. pylori using electrical DNA sensing

描述（由申请人提供）：本提案描述了开发 利用DNA固有特性的新型生物传感器： 电传导dsDNA的导电能力， 结合测量这种传导的能力（<100bp）， 特定的DNA序列，将被利用来生产传感器， 前所未有的灵敏度和便携性。常规检测方法 DNA阵列上的序列采用光学检测， 需要检测临床样品中的微生物物种，而无需广泛的 处理.本计画的目标是建构一个奈米尺度的DNA感测器 具有电检测，可以灵敏且准确地检测 幽门螺杆菌是一种与胃溃疡和癌症有关的细菌， 临床样本。该项目的具体目标包括：（1） 演示了电子DNA传感的原理验证 用合成DNA分子模拟一个独特的H pylori基因，和（2） 检测H.幽门螺杆菌临床样本使用电子DNA检测。

DESCRIPTION (provided by applicant): This proposal describes the development of a new class of biosensors exploiting an intrinsic property of DNA: electrical conduction. The capability of dsDNA to conduct electricity, in combination with the ability to measure this conduction in short (<100bp) and specific DNA sequences, will be harnessed to produce sensors with unprecedented sensitivity and portability. Conventional methods of detecting sequences on DNA arrays employ optical detection, and lack the sensitivity required to detect microbial species in clinical samples without extensive processing. The goal of this project is to construct a nanoscale DNA sensor with electrical detection that can sensitively and accurately detect Helicobacter pylori, a bacterium associated with gastric ulcers and cancer, in clinical samples. The specific aims of this project include (1) the demonstration of proof-of-principle for electrical DNA sensing carried out with synthetic DNA molecules modeling a unique H. pylori gene, and (2) the detection of H. pylori in clinical samples using electrical DNA detection.