Serial analysis of gene expression in P.falciparum

描述：（申请人提供）：高通量方法的出现 与疟疾基因组一起分析全球基因表达 项目为我们进一步了解 疟疾寄生虫的基本生物学和毒力。系列分析 基因表达（SAGE）特别适用于疟疾系统， 关于基因表达和许多基因的已知很少， 测序项目的功能未知。SAGE是一个非常强大的工具 同时定量分析mRNA转录本 从一个给定的细胞群体的配置文件，允许发现新的基因。 这是第一次，现在可以检查所有的反应， 基因的刺激，如药物治疗或体内免疫压力。 将基因组序列与功能联系起来，并最终与生物学联系起来， 挑战.在其他系统中工作，如酿酒酵母和人类 已经证明了对生长条件、细胞生长和细胞增殖的变化的多基因反应。 周期变化，对药物治疗的反应以及不同癌症的反应。等 基因表达模式已被证明在识别基因网络中是有用的 在共同的控制下，并导致识别新的途径， 用于药物和疫苗的研发这些方法允许 在没有身份的先验知识的情况下对新基因进行功能分析。的 这项工作的目的是通过以下方法来检查恶性疟原虫的转录 确定寄生虫预测的7000个基因中的哪一个被表达 在寄生虫生命周期的不同阶段或在不同条件下， 如在药物存在或免疫压力下。此表达式 然后，信息将用于选择特定的基因进行进一步的功能研究。 分析并识别基因表达的网络或模式， 用于鉴定控制疟原虫中转录的元件 恶性疟原虫。这项工作的结果将作为科学研究的资源。 社区，无论是在功能分析方面， 新基因的功能。这些目标的实现， 基因表达系列分析（SAGE）技术的优化， 恶性疟原虫;基因表达网络的鉴定和 参与应答特异性转录的顺式元件的表征 控制和使用基因表达谱和网络，以确定新的 药物和疫苗开发的目标。

DESCRIPTION: (provided by the applicant): The advent of high-throughput methods for the analysis of global gene expression together with the Malaria Genome Project open up new opportunities for furthering our understanding of the fundamental biology and virulence of the malaria parasite. Serial analysis of gene expression (SAGE) is particularly well suited for malarial systems, as little is known about gene expression and the many of genes identified in the sequencing project are of unknown function. SAGE is an extremely powerful tool with which to simultaneously and quantitatively analyze mRNA transcript profiles from a given cell population, allowing for the discovery of new genes. For the first time, it is now possible to examine the response of all of the genes to stimuli such as drug treatment or immune pressure in vivo. Relating genomic sequence to function and ultimately to the biology is the next challenge. Work in other systems such as Saccharomyces cerevisiae and humans has demonstrated a multigenic response to changes in growth conditions, cell cycle changes, responses to drug treatment and in different cancers. Such patterns of gene expression have proven useful in identifying networks of genes under common control and led to the identification of novel pathways which can be exploited for drug and vaccine development. Such approaches allow for the functional analysis of new genes without prior knowledge of the identity. The goal of this work is to examine transcription in Plasmodium falciparum through the determination of which of the parasite's predicted 7000 genes are expressed at various stages of the parasite life cycle or under different conditions such as in the presence of drug or under immune pressure. This expression information will then be used to choose specific genes for further functional analysis and to identify networks or patterns of gene expression that will be useful in identifying elements which control transcription in Plasmodium falciparum. Results from this work will serve as a resource of the scientific community, both in term of functional analysis and in the identification of functions of new genes. These goals will be achieved the development and optimization of the Serial Analysis of Gene Expression (SAGE) technology for Plasmodium falciparum; identification of networks of gene expression and characterization of cis elements involved in response-specific transcriptional control and the use of gene expression profiles and networks to identify new targets for drug and vaccine development.