CORE--TISSUE CULTURE

上皮细胞培养有助于确定病理生理学。 Cf.建立了组织采购和细胞培养核心 北卡罗来纳大学(UNC)于1984年成立，由 囊性纤维化基金会，为囊性纤维化提供标准化细胞培养 研究人员。核心支持了北卡罗来纳大学囊性纤维化的项目 自1988年成立研究中心以来，产量和能力都有所提高 以满足日益增长的研究需求。组织培养核心将支持 SCOR通过三个主要功能进行项目设计。 1.纸巾采购。 。取正常、疾病对照和囊性病变的鼻腔和肺组织。 纤维化的人作为呼吸道上皮细胞的来源。 。在诊断、人口统计和临床方面描述供者的特征 对研究用途至关重要的功能。 2.呼吸道上皮细胞的分离培养。 。分离人、灵长类动物大小呼吸道上皮细胞 和啮齿动物的肺。 。从Define准备和维护原代上皮细胞培养 呼吸道区域。 。从传代的或冷冻保存的和 根据研究人员的需要在基质上解冻细胞。 。利用基因敲除技术制备和提供呼吸道上皮细胞系 老鼠。 3.细胞培养技术的发展。 。优化上皮细胞培养条件以复制基因表达， 细菌的形态分化、离子和粘液分泌功能 正常和CF大、小气道。 。产生基因操纵的、分化良好的初级呼吸道 上皮细胞培养。 。提供评估呼吸道所需的非常高湿度的培养环境 表面液体在没有蒸发伪影的情况下的地形。

Epithelial cell cultures are instrumental in defining the pathophysiology of CF. A Tissue Procurement and Cell Culture Core was established at the University of North Carolina (UNC) in 1984, under the auspices of the Cystic Fibrosis Foundation, to provide standardized cell cultures to CF researchers. The Core has supported projects of the UNC Cystic Fibrosis Research Center since 1988, and has increased its output and capabilities to meet growing research demands. The Tissue Culture Core will support the SCOR projects through three major functions. 1. Tissue procurement. . Obtain nasal and lung tissue from normal, disease control, and cystic fibrosis humans as sources of airway epithelial cells. . Characterize donors with respect to diagnosis, demographic, and clinical features essential to research uses. 2. Airway epithelial cell isolation and culture. . Isolate epithelial cells from large and small airways of human, primate and rodent lungs. . Prepare and maintain primary epithelial cell cultures from define airway regions. . Prepare differentiated cultures from passaged, or cryopreserved and thawed cells on substrates as dictated by investigator needs. . Prepared and provide airway epithelial cell lines from gene knockout mice. 3. Cell culture technical development. . Optimize epithelial culture conditions to replicate the gene expression, morphological differentiation, and ion and mucus secretory functions of normal and CF large and small airways. . Produce genetically manipulated, well-differentiated primary airway epithelial cell cultures. . Provide very high humidity culture environments needed to assess airway surface liquid topography in the absence of evaporation artifacts.