CORE--CELLULAR AND MOLECULAR BIOLOGY FACILITY

多器官衰竭（MOF）发生在很大一部分创伤受害者中，它仍然是外科重症监护病房首次复苏后死亡的主要原因。为了了解胃肠道对该综合征发病机制的贡献，本中心的个别项目提出，必须采用专门的细胞和分子生物学方法、试剂、设备和方法。细胞和分子生物学核心的主要目标是开发并向创伤中心的研究人员提供分子试剂、复杂的方法、专用设备和培训，以检测、定位和定量涉及MOF发病机制的特定基因产物。核心将维持一个集中的专业知识和设备设施，以促进中心的项目，保持方法的标准化，并确保质量控制。重组DNA方法、原位杂交和mRNA定量方法，包括“实时”定量PCR，将用于评估mRNA的分布和丰度。免疫印迹、免疫组织化学、共聚焦免疫荧光显微镜和免疫电镜将用于鉴定组织中靶蛋白的丰度和细胞和亚细胞定位。中心的工作人员将为中心成员提供适合他们研究的细胞和分子策略的建议和培训，并协助他们进行数据解释和分析。核心将被中心应用程序中的每个项目使用。

Multiple organ failure (MOF) occurs in a substantial portion of trauma victims, and it remains the leading cause of death after initial resuscitation in surgical intensive care units. To understand the contributions of the gastrointestinal tract to the pathogenesis of this syndrome is proposed in the individual projects of this center, specialized cellular and molecular biological approaches, reagents, equipment, and methods must be employed. The principal goal of the Cellular and Molecular Biology Core is to develop and provide to Trauma Center investigators molecular reagents, sophisticated methodology, specialized equipment, and training for the detection, localization, and quantitation of specific gene products involved in the pathogenesis of MOF. The Core will maintain a centralized facility of expertise and equipment to facilitate the projects of the Center, maintain standardization of methods, and insure quality control. Recombinant DNA methods, in situ hybridization, and mRNA quantitation methods, including "real-time" quantitative PCR, will be used to assess mRNA distribution and abundance. Immunoblotting, immunohistochemistry, confocal immunofluorescence microscopy, and immunoelectron microscopy will be utilized to identify the abundance and the cellular and subcellular localization of target proteins in tissues. The staff of the Core will advice and train Center members in the cellular and molecular strategies appropriate for their studies, and assist them in data interpretation and analysis. The Core will be utilized by every project in the Center application.