Application of novel cellular target engagement technologies to interrogate the IL23R-JAK/TyK2-STAT signalling pathway

许多生物过程是基于内源性配体的结合和其他形成信号级联的蛋白质-蛋白质相互作用。对于一些细胞机制来说，由于可用于帮助去卷积的有限的工具，理解这些信号级联的核心组件和相互作用仍然是难以捉摸的。这项研究试图利用NanoBRET示踪器和蛋白质-蛋白质相互作用(PPI)技术来定位和更好地了解细胞环境中已知的信号通路(IL23R/JAK家族/STAT)的元件。

Many biological processes are predicated on the binding of endogenous ligands and other protein-protein interactions that form part of a signaling cascade. Understanding the core components and interactions of these signaling cascades is still elusive for a number of cellular mechanisms with limited tools available to aid deconvolution. This study seeks to utilise the NanoBRET Tracer and protein-protein interaction (PPI) technology to target and better understand elements of a known signaling pathway (IL23R/JAK family/STAT) in a cellular context.