CORE--MOLECULAR BIOLOGY

分子生物学核心设施（核心A）是一个共享资源，为拟议的达特茅斯SBRP计划的七个单独研究项目中的六个提供并整合了许多相关的分子生物学和生物化学服务。该核心的总体目标是以低成本、及时和高质量的方式提供所需的分子生物学服务，在中央机构支持的设施中使用现有的和摇摆的最先进的设备和全职技术人员，并为各个项目提供高水平的技术专长和咨询。该核心的具体目标是：1）提供大分子（DNA，RNA，肽，蛋白质）测序和合成服务，包括标准和定制寡核苷酸合成;自动化循环DNA测序和合成服务，包括标准和定制寡核苷酸合成自动化循环DNA测序，肽合成和纯化，自动化蛋白质测序; LCQ-大分子质谱分析; 2）提供图像生成和分析服务，包括磷光成像、数字光密度测定和分析、X光胶片处理和流式细胞术;以及3）为分子生物标志物的开发提供支持，包括差异显示分析、核糖核酸酶保护测定分析、基于Affymetrix的cDNA/基因“芯片”技术;用户生成的定制cDNA阵列功能;新鉴定序列的克隆和测序支持;基于计算机的序列分析支持;遗传多态性分析;潜在蛋白质和核酸生物标志物的方法开发，用于生态学和流行病学试验以及更基础的研究项目中的进一步假设检验。对这一核心的支持被认为是至关重要的，是这一方案项目的总体目标和成功的核心。

The Molecular Biology Core Facility (Core A) is a shared resource that provides and integrates a number of related molecular biology an biochemistry services for six of the seven individual research projects of the proposed Dartmouth SBRP Program. The overall goal of this core is to provide needed molecular biology services in a low-cost, timely and high quality manner, using existing and pendular state-of-the-art equipment and full-time technical personnel in a central institutionally supported facility, and to provide high level technical expertise and advice to individual projects and advice to individual projects. The Specific Aims of this core are to 1) provide macromolecular (DNA, RNA, peptides, proteins) sequencing and synthesis services including standard and custom oligonucleotide synthesis; automated cycle DNA sequencing and synthesis services including standard and custom oligonucleotide synthesis automated cycle DNA sequencing, peptide synthesis and purification, automated protein sequencing; LCQ-Mass spectral analysis of macromolecules; and HPLC and PCR support services; 2) provide image generation and analysis services including phosphorimaging, digital densitometry and analysis; x-ray film processing; and flow cytometry; and 3) provide support for development of molecular biomarkers including differential display analysis, ribonuclease protection assay analysis; Affymetrix-based cDNA/ gene "chip" technology; user- generated custom cDNA array capabilities; cloning and sequencing support for newly identified sequences; support for computer-based sequence analysis; genetic polymorphism analysis; method development for potential protein and nucleic acid-based biomarkers for application in ecological and epidemiological trials as well as for further hypothesis testing in the more basic research projects. Support for this core is seen as vital and central to the overall goals and success of this program project.