Molecular Biology

分子生物学

• 批准号: 6989448
• 负责人: Joshua W Hamilton
• 金额: $ 5.36万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-02 至 2008-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6989448
• 关键词: high performance liquid chromatography; mass spectrometry; molecular biology; neoplasm /cancer; peptide chemical synthesis; protein purification; protein sequence; proteomics

The Norris Cotton Cancer Center (NCCC) Molecular Biology and Proteomics (MB&P) Shared Resource has been operating since 1995. It provides state-of-the-art molecular biology support including DNA and RNA oligonucleotide synthesis, DNA sequencing, fragment analysis, traditional and real-time PCR services, and various imaging support services for the NCCC membership. This laboratory also synergizes with the Bioinformatics Shared Service and the DNA Microarray Shared Service in providing seamless support for the investigator who is applying these tools for genomic level analyses. Recently, the MB&P Shared Resource completed the development of a full range of proteomics support services, including peptide synthesis and purification, protein sequencing, 2D gel analysis of proteins, and mass analysis of proteins using two recently acquired mass spectrometers, a MALDI-TOF-MS and an HPLC-ESI-Q-MS. With this increase in services has come a related increase in staff specialized and trained to accommodate the intellectual and technical needs of the NCCC scientific community. The MB&P Shared Resource currently provides 38 NCCC members and their laboratories with services, which totaled $108,000 in charge-backs for FY 2002. The overall goal of this Shared Resource is to continue to provide NCCC researchers with state-of-the-art technical support and services for their cancer research at as low a rate as possible so that they can continue to successfully meet their research objectives individually and collectively.

诺里斯棉花癌症中心(NCCC)分子生物学和蛋白质组学(MB&P)分享 自1995年以来，资源公司一直在运营。它提供了最先进的分子生物学支持 包括DNA和RNA寡核苷酸合成、DNA测序、片段分析、传统和实时PCR服务，以及为NCCC成员提供的各种成像支持服务。该实验室还与生物信息学共享服务和DNA微阵列共享服务协同工作，为应用这些工具进行基因组水平分析的研究人员提供无缝支持。最近，MB&P共享资源完成了一整套蛋白质组学支持服务的开发，包括多肽合成和纯化、蛋白质测序、蛋白质的二维凝胶分析以及使用最近获得的两个质谱仪--MALDI-TOF-MS和LC-ESI-Q-MS对蛋白质的质量分析。随着服务的增加，专门和培训的工作人员也相应增加，以适应NCCC科学界的智力和技术需求。MB&P共享资源目前提供38个NCCC成员及其 提供服务的实验室，2002财年的按存储容量使用计费总计10.8万美元。这一共享资源的总体目标是继续以尽可能低的比率为NCCC研究人员提供最先进的癌症研究技术支持和服务，使他们能够继续成功地单独和集体实现他们的研究目标。