Molecular Biology

诺里斯棉花癌症中心（NCCC）分子生物学和蛋白质组学（MB&P）共享 自1995年以来一直在运营。它提供最先进的分子生物学支持 包括DNA和RNA寡核苷酸合成、DNA测序、片段分析、传统和实时PCR服务以及为NCCC会员提供的各种成像支持服务。该实验室还与生物信息学共享服务和DNA微阵列共享服务协同合作，为应用这些工具进行基因组水平分析的研究人员提供无缝支持。最近，MB&P共享资源完成了全方位蛋白质组学支持服务的开发，包括肽合成和纯化，蛋白质测序，蛋白质的2D凝胶分析，以及使用两台最近获得的质谱仪进行蛋白质的质量分析，MALDI-TOF-MS和HPLC-ESI-Q-随着服务的增加，专业化和训练有素的工作人员也相应增加，以满足NCCC科学界的知识和技术需求。MB&P共享资源目前提供38个NCCC成员及其 2002财政年度的收费总额为108，000美元。该共享资源的总体目标是继续为NCCC研究人员提供最先进的技术支持和服务，以尽可能低的速度进行癌症研究，使他们能够继续成功地实现他们的研究目标。

The Norris Cotton Cancer Center (NCCC) Molecular Biology and Proteomics (MB&P) Shared Resource has been operating since 1995. It provides state-of-the-art molecular biology support including DNA and RNA oligonucleotide synthesis, DNA sequencing, fragment analysis, traditional and real-time PCR services, and various imaging support services for the NCCC membership. This laboratory also synergizes with the Bioinformatics Shared Service and the DNA Microarray Shared Service in providing seamless support for the investigator who is applying these tools for genomic level analyses. Recently, the MB&P Shared Resource completed the development of a full range of proteomics support services, including peptide synthesis and purification, protein sequencing, 2D gel analysis of proteins, and mass analysis of proteins using two recently acquired mass spectrometers, a MALDI-TOF-MS and an HPLC-ESI-Q-MS. With this increase in services has come a related increase in staff specialized and trained to accommodate the intellectual and technical needs of the NCCC scientific community. The MB&P Shared Resource currently provides 38 NCCC members and their laboratories with services, which totaled $108,000 in charge-backs for FY 2002. The overall goal of this Shared Resource is to continue to provide NCCC researchers with state-of-the-art technical support and services for their cancer research at as low a rate as possible so that they can continue to successfully meet their research objectives individually and collectively.