DARTMOUTH COL COBRE: PROTEOMICS FACILITY CORE

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在给定时间在细胞中表达的表达蛋白质的整个谱被描述为蛋白质组，与基因组，编码蛋白质和其他基因产物的DNA不同。 单个基因可以表达许多不同形式的蛋白质，并且蛋白质的转录后修饰可以导致进一步的多样性，使得蛋白质组与基因组或甚至mRNA表达的基因组不同，并且可以在特征和组成上与基因组或甚至mRNA表达的基因组完全不同。 蛋白质组学涉及生物系统中表达的蛋白质组的定性和定量分析，并且需要高度专业化的培训和仪器来完成。因此，蛋白质组学核心是现代生物医学研究企业的重要组成部分。 蛋白质组学是我们的COBRE计划的五个初始研究项目中的四个的主要组成部分，也是该计划高级导师研究的组成部分。我们的目标是通过COBRE资助在达特茅斯建立一个最先进的蛋白质组学设施核心，提供一系列完整的蛋白质组学相关服务。 这包括招聘一名教师蛋白质组学专家到达特茅斯，翻新达特茅斯新生的分子生物学和蛋白质组学核心设施空间，培训核心专业人员，并收购最先进的仪器。 在第三年，我们成功地招募了一名蛋白质组学专家，开始对核心设施进行翻新，开始购买仪器，并开始对核心的操作和工作人员进行全面的培训和方法审查。 我们在本年度继续这一进程，并取得了下文所述的巨大成功。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The entire spectrum of expressed proteins expressed in a cell at a given time is described as the proteome, as distinguished from the genome, the DNA that codes for proteins and other gene products. A single gene can express many different forms of proteins, and post-transcriptional modification of proteins can result in further variety such that the proteome is distinct from, and can be quite different in character and makeup from the genome or even the mRNA expressed genome. Proteomics involves the qualitative and quantitative analysis of the expressed proteome in biological systems and required highly specialized training and instrumentation to accomplish Thus, a proteomics core is an important component of a modern biomedical research enterprise. Proteomics is a major component of four of the five initial research projects of our COBRE program, as well as a component of the research of the senior mentors of this program. Our goal is to develop through the COBRE grant a state-of-the-art Proteomics Facility Core at Dartmouth with a complete series of proteomics-related services. This includes recruitment of a faculty proteomics expert to Dartmouth, renovation of the nascent Molecular Biology & Proteomics Core facility space at Dartmouth, training of professional staff for the core, and acquisition of state-of-the-art instrumentation. In Year Three we successfully recruited a proteomics expert, began renovations of the core facility, began acquisition of instruments, and began a comprehensive training and methodology review of the core's operations and staff. We have continued this process in the current year with great success as described below.