DARTMOUTH COL COBRE: PROTEOMICS FACILITY CORE

达特茅斯 COL COBRE：蛋白质组学设施核心

• 批准号: 7382079
• 负责人: Joshua W Hamilton
• 金额: $ 26.06万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7382079
• 关键词: DARTMOUTH; COL; COBRE; PROTEOMICS; FACILITY

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Proteomics is a major component of four of the five initial research projects of our COBRE program, as well as a component of the research of the senior mentors of this program. Our goal is to develop a Proteomics Facility Core with a complete series of proteomics-related services. In the shortened year 01 we began to develop and expand the Proteomics Core's instrumentation and methodologies with the acquisition of a Biacore X SPR biosensor to perform surface plasmon resonance studies (SPR). We also began the planning process for renovation of the core facilities using the A&R funds from this COBRE award. In year 02 we have continued to expand and develop proteomics related services and methodologies for our investigators, including 2D gel electrophoretic and PF2D liquid separation techniques for complex protein samples and improvements to the ESI-MS injection system. We also successfully recruited a bioinformatics faculty (J. Moore) as part of the COBRE program who will assist with analysis of proteomics data. Projects 2 and 3 have begun using proteomics tools in their research with the core's support and assistance. We are now working with Projects 1 and 4 to discuss their proteomics related research and develop appropriate methods and tools for their use. We submitted the finalized renovation plans to NIH COBRE staff in year 02 for final approval, and began the process of obtaining local A&R permitting as well as contracting for final design. We have now begun the renovation process, with completion nearing for one of the three phases of the project and initiation of the other two phases. We anticipate completion of these phases over the next 4-6 months.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。蛋白质组学是我们 COBRE 项目五个初始研究项目中四个的主要组成部分，也是该项目高级导师研究的组成部分。我们的目标是开发一个蛋白质组学设施核心，提供一系列完整的蛋白质组学相关服务。在缩短的 01 年，我们开始开发和扩展蛋白质组学核心的仪器和方法，购买了 Biacore X SPR 生物传感器来执行表面等离子共振研究 (SPR)。我们还开始利用 COBRE 奖项的 A&R 资金改造核心设施的规划过程。在第二年，我们继续为我们的研究人员扩展和开发蛋白质组学相关服务和方法，包括用于复杂蛋白质样品的 2D 凝胶电泳和 PF2D 液体分离技术以及 ESI-MS 注射系统的改进。我们还成功招募了一名生物信息学教师 (J. Moore) 作为 COBRE 计划的一部分，他将协助分析蛋白质组数据。在核心的支持和协助下，项目 2 和 3 已开始在其研究中使用蛋白质组学工具。我们现在正在与项目 1 和 4 合作，讨论他们的蛋白质组学相关研究，并为其使用开发适当的方法和工具。我们在第二年向 NIH COBRE 工作人员提交了最终的改造计划以获得最终批准，并开始获得当地 A&R 许可以及最终设计合同。我们现已开始翻修工程，该项目的三个阶段之一即将完成，另外两个阶段也已启动。我们预计这些阶段将在未来 4-6 个月内完成。