Core--Hazard identification

与所有多组件计划项目一样，需要跨多个项目的公共服务。该核心的目标是提供生物化学，细胞，分子，分子，分析和人类研究，将用于研究项目和技术示范核心。危害识别核心（HIC）将为研究项目提供信息，以更好地评估环境对周围人群的风险。该核心的具体目标是在三个领域提供研究支持：毒性评价、分析和人体研究。毒性评价：本节将提供细胞毒性筛选（细胞和组织切片），作为所有研究项目和技术演示核心的遗传毒性筛选（艾姆斯试验和细胞/切片中的计划外DNA合成）。此外，我们将与大学合作，建立一个基因阵列服务，该服务将对我们所有研究的样本中的改变基因进行评估。分析部分：由于我们的大多数项目都是研究砷在环境中的处理及其在动物和人类中的命运/毒性，我们将提供砷分析。需要更敏感的技术来检测各种基质中的砷（总）和砷化学物种，因为我们的调查集中在低，环境相关浓度的砷。使用HPLC-氢化物发生-原子荧光光谱法和电感耦合等离子体质谱法，本节将处理所有研究和TechDemo项目所需的所有砷分析和推测。人体研究：我们将继续进行关于人体金属（如砷和汞）负荷的公认的人体研究。这些调查人员是我们超级基金申请的一个重要组成部分，因为他们使我们能够接触到因处理金属而被定性的暴露人群。这些研究提供了一个机会，将我们实验室开发的信息和分析转化为帮助评估人类风险评估。将生成毒性评价和分析章节，其中生物标志物可用于人体研究章节，以更好地评估砷暴露风险。

As with all multi-component Program Projects there are common services needed that span several projects. The objective of this Core is to provide biochemical, cellular, molecular, molecular, analytical, and human studies that will be utilized by the Research Projects and Technology Demonstration Core. The Hazard Identification Core (HIC) will provide information for the research projects to better assess the risk of the environmental to the surrounding populace. The Specific Aims of this Core will be to provide research support in three areas; toxicity evaluation, analytical, and human studies. Toxicity Evaluation: This section will provide cytotoxicity screen (cells and tissue slices) as a genotoxic screen (Ames test and unscheduled DNA synthesis in cells/slices) for all the research projects and the Tech Demo Core. In addition we will work with the University to have a gene array service which will provide an evaluation of altered genes in samples from all of our studies. Analytical Section: Since the majority of our projects are studying the processing of arsenic in the environment and its fate/toxicity in animals and humans we will provide arsenic analyses. More sensitive techniques are needed to detect arsenic (total) and arsenic chemical species in various matrixes since our investigations have focused on low, environmentally relevant concentrations of arsenic. Using HPLC-hydride generation- atomic fluorescence spectroscopy and inductively coupled plasma mass spectrometry this section will handle all the arsenic analysis and speculation that will be required from all the research and TechDemo projects. Human Studies: We will continue our well-recognized human studies concerning body burden of metals (e.g. As and Hg). These investigators are an important component of our Superfund application since they allow us access to exposed populations who have been characterized for their handling of metals. These studies offer an opportunity to translate the information and analyses developed in our laboratories to help evaluate human risk assessment. Both the Toxicity Evaluation and Analytical Sections will be generated that biomarkers that can be used in the Human Studies Section to better assess the risk of exposure to arsenic.