CORE--TRANSGENIC MICE

(改编自申请者摘要)：该核心将具有三个功能： 1)将计划与成熟和高度成功的公司对接， 阿尔伯特·爱因斯坦博物馆提供转基因和基因打靶设备 医学院。在这个设施中，将产生转基因小鼠 使用Core B提供的DNA结构。此功能将主要用于 为调查人员提供标签、身份证明和 创始人及其后代的记录保存。2)识别 表达所需的血红蛋白或红细胞转运蛋白的创始人， 描述它们的特征，并为它们的繁殖策略提供帮助 创始人。这些措施包括，将它们培育到转基因小鼠中表达 AlphaH Beta、Alpha淘汰赛、Beta淘汰赛或其他 可获得转基因和基因敲除品系。3)培育转基因小鼠 将在项目1(两只动物)中进行识别和表征 镰状细胞病的器官损伤模型)；项目2(转基因 同时表达HBc、HBs和HBc；项目6(转基因小鼠 表达人K：c1共转运)。

(Adapted from Applicant's Abstract): This core will have three functions: 1) Interface the Program with the well established and highly successful, Transgenic and Gene Targeting Facility available at the Albert Einstein College of Medicine. In this facility transgenic mice will be generated with DNA constructs supplied by Core B. This function will be mainly to provide assistance to investigators in the labeling, identification and record keeping of the founders and their future generations. 2) Identify founders expressing the desired hemoglobins or red cell transporters, characterize them and provide assistance with breeding strategies of the founders. These include, breeding them to the transgenic mice expressing alphaH BetaS, the alpha-knockout, the Beta-knockouts, or to other transgenic and knockout lines available. 3) Breed transgenic mice which will be identified and characterized for use in Project 1 (Two animal models of organ damage in sickle cell disease); Project 2 (Transgenic expressing HbC and HbS and HbC simultaneously; Project 6 (Transgenic mice expression human K:C1 co-transport).