STAFF PRIORITY TIME XRAY ABSORPTION SPECTROSCOPY OF SULFITEOXIDASE

氧化还原电位法、电子顺磁法、 共振（EPR）谱和Mo K边X射线吸收 光谱学已经应用于野生型人类和一系列 突变的人亚硫酸盐氧化酶，包括两个临床突变体。 的 结合氧化还原电位和EPR光谱，使我们能够 确定待检测氧化还原中毒样品的精确条件 通过X射线吸收光谱，我们可以检查活性 Mo（VI）、Mo（V）和Mo（IV）氧化态的位置结构。 已经收集了大量样本的广泛数据， 详细分析工作正在进行中。 我们希望能够找出 几种氨基酸在催化循环中的功能作用 靠近钼的活性部位。

The techniques of redox potentiometry, electron paramagnetic resonance (EPR) spectroscopy and Mo K-edge X-ray absorption spectroscopy have been applied to wild-type human and a series of mutant human sulfite oxidases, including two clinical mutants. The combination of redox potentiometry and EPR spectroscopy has allowed us to define precise conditions for redox poising samples to be examined by x-ray absorption spectroscopy, allowing us to examine the active site structures of the Mo(VI), Mo(V) and the Mo(IV) oxidation states. Extensive data have been collected on a large number of samples and detailed analysis is now underway. We expect to be able to identify the functional role within the catalytic cycle for several amino acids near to the molybdenum active site.