EXXON PRT TIME SULFUR K EDGE SPECT IN SITU PROBE OF SULFUR SPECIES IN BACTERIA

微生物脱硫提供了一种潜在的新方法 以最小的环境条件脱除燃料油中的硫化物 影响(Gray等人，(1996)自然生物技术14,1705-1709)。 在分离个体基因方面取得了进展 酶在脱硫途径中的作用，并研究它们在 重组大肠杆菌。我们正在使用硫磺K边光谱来 跟踪这些微生物对燃料油的脱硫作用 试图了解它们在复杂混合物中的底物专一性。

Microbial desulfurization offers a potential new approach to removing sulfur compounds from fuel oils with minimal environmental impacts (Gray et al., (1996) Nature Biotechnology 14, 1705-1709). Progress has been made in isolating the genes for the individual enzymes in the desulfurization pathway, and studying their function in recombinant E. coli. We are using sulfur K-edge spectroscopy to follow the desulfurization of fuel oils by these organisms in an attempt to understand their substrate specificity in complex mixtures.